Anti-CERK monoclonal antibody (DCABH-10989) Made to order

Rabbit anti-Human CERK monoclonal antibody for WB, ELISA

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
A synthetic peptide of human CERK is used for rabbit immunization.
Conjugate
Unconjugated

Target


Alternative Names
CERK; ceramide kinase; dA59H18.2; dA59H18.3; DKFZp434E0211; FLJ21430
Entrez Gene ID
UniProt ID

Product Background


Gene summary
CERK (Ceramide Kinase) is a Protein Coding gene. Diseases associated with CERK include retinitis pigmentosa. Among its related pathways are Metabolism and Integrated Breast Cancer Pathway. GO annotations related to this gene include magnesium ion binding and diacylglycerol kinase activity. An important paralog of this gene is SPHK2. CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al. , 2006 [PubMed 16488390]).
Antigen Description
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al. , 2006 [PubMed 16488390]). Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. In enzymology, a ceramide kinase, also abbreviated as CERK, (EC 2. 7. 1. 138) is an enzyme that catalyzes the chemical reaction:Production of C-1-P bolsters cell survival and proliferation. It has been shown that C-1-P promotes DNA synthesis in fibroblasts. C-1-P also prevents apoptosis by inhibiting the caspase-9/caspase-3 pathway and preventing DNA fragmentation in macrophages. This is thought to occur via C-1-P interacting with and blocking functionality of acid sphingomyelinase. This results in diminished ceramide production, which precludes apoptosis. Recently, phosphorylation of ceramide via CERK has been shown to stimulate myoblast proliferation. It was demonstrated that C-1-P perpetuates the phosphorylation of glycogen synthase kinase-3 β and retinoblastoma protein, which contributes to transition from the G1 phase to M phase of the cell cycle. Additionally, production of C-1-P appears to result in increased expression of Cyclin D. CERK has demonstrated an ability to activate phosphatidylinositol 3-kinase/Akt (PI3K/Akt), ERK1/2, and mTOR. CERKs ability to produce signaling molecules that facilitate the activation of cell proliferation as well as its interaction with PI3K/Akt, and mTOR indicate that disregulated CERK expression may lead to cancer.
Pathway
Glycosphingolipid metabolism, organism-specific biosystem; Integrated Breast Cancer Pathway, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem; Sphingolipid metabolism, conserved biosystem.

Citations


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References


Murakami, M; Ito, H; et al. ATRA inhibits ceramide kinase transcription in a human neuroblastoma cell line, SH-SY5Y cells: the role of COUP-TFI. JOURNAL OF NEUROCHEMISTRY 112:511-520(2010).
Mitsutake, S; Igarashi, Y; et al. Calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium sensor. JOURNAL OF BIOLOGICAL CHEMISTRY 280:40436-40441(2005).

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