Anti-Human CD3D monoclonal antibody (DCABH-231)

Rabbit Anti-Human CD3D monoclonal antibody for WB, ICC, IHC, IP, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
KK19-08
Species Reactivity
Human
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:100-1:500; IHC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of CD3D on Jurkat cells lysates using anti-CD3D antibody at 1/1,000 dilution.
Fig2: ICC staining CD3D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD3D in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining CD3D in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3D antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD3D antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3D antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of Raji cells with CD3D antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
CD3 antigen delta subunit; CD3 delta; CD3d; CD3d antigen delta polypeptide (TiT3 complex); CD3d molecule delta (CD3-TCR complex); CD3D_HUMAN
Entrez Gene ID
UniProt ID

Citations


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References


Munoz-Ruiz, M; Perez-Flores, V; et al. Human CD3 gamma, but not CD3 delta, haploinsufficiency differentially impairs gamma delta versus alpha beta surface TCR expression. BMC IMMUNOLOGY 14:-(2013).
Hu, QH; Pan, ZM; et al. New alleles of chicken CD8 alpha and CD3d found in Chinese native and western breeds. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 120:223-233(2007).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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