Anti-Cd16/Cd32 monoclonal antibody (CABT-45660RM)

Rat anti-Mouse Cd16/Cd32 monoclonal antibody for FC

Additional Formats Available

Specifications


Host Species
Rat
Antibody Isotype
IgG2b
Clone
FCR4G8
Species Reactivity
Mouse
Immunogen
PU5 1.8 IOE7 Balb/c mouse cell line.
Conjugate
Unconjugated

Applications


Application Notes
Flow Cyt: 1/25 - 1/50;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
FCGR3; Fc receptor, IgG, low affinity III; CD16; low affinity immunoglobulin gamma Fc region receptor III; Fc gamma receptor III; Fcg receptor III
Entrez Gene ID
UniProt ID
P08101

Product Background


Antigen Description
The function about FCGR3 antigen include IgG binding; IgG receptor activity.
Pathway
FCGR activation; Fcgamma receptor (FCGR) dependent phagocytosis; Immune System; Innate Immune System; Leishmaniasis; Osteoclast differentiation; Phagosome; Regulation of actin dynamics for phagocytic cup formation.

Citations


Have you cited CABT-45660RM in a publication? Let us know and earn a reward for your research.

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Rat IgG2b Isotype Control DAGIC1371 FC EIA IHC Rat PDF Inquiry

References


Identification of genes underlying the enhancement of immunity by a formula of lentinan, pachymaran and tremelia polysaccharides in immunosuppressive mice

SCIENTIFIC REPORTS

Authors: Luo, Xia; Huang, Shaowei; Luo, Shuang; Liao, Haifeng; Wang, Yuanyuan; Deng, Xiangliang; Ma, Fangli; Ma, Chung Wah; Zhou, Lian

The efficacy of polysaccharides is widespread, especially in immune regulation. However, the genetic basis of the changes in polysaccharides regulating immunity is unclear. To obtain genome-wide insights into transcriptome changes and regulatory networks, we designed a polysaccharide formula, comprising lentinan, pachymaran and tremelia, to increase the availability of their optimized active sites. In this case, we focused on a model of immunosuppression to investigate genes by digital gene expression (DGE) tag profiling in T and B cells. These genes were further validated by qRT-PCR and Western blot experiments. Consequently, polysaccharide formula treatment helped to recover the expression of immune-related genes, including CADM1, CCR2, IGLL1, LIGP1, and FCGR3, FCGR2 in B cells, as well as S100A8, S100A9, ChIL3, MMP8 and IFITM3 in T cells. These results suggest that treatment with polysaccharides improves the immunity of immunosuppressive mice by regulating genes associated with T and B cell functions.

Experimental crescentic glomerulonephritis: a new bicongenic rat model

DISEASE MODELS & MECHANISMS

Authors: D'Souza, Zelpha; McAdoo, Stephen P.; Smith, Jennifer; Pusey, Charles D.; Cook, H. Terence; Behmoaras, Jacques; Aitman, Timothy J.

Crescentic glomerulonephritis (CRGN) is a major cause of human kidney failure, but the underlying mechanisms are not fully understood. Wistar Kyoto (WKY) rats are uniquely susceptible to CRGN following injection of nephrotoxic serum, whereas Lewis (LEW) rats are resistant. Our previous genetic studies of nephrotoxic nephritis (NTN), a form of CRGN induced by nephrotoxic serum, identified Fcgr3 and Jund as WKY genes underlying the two strongest quantitative trait loci for NTN phenotypes: Crgn1 and Crgn2, respectively. We also showed that introgression of WKY Crgn1 or Crgn2 individually into a LEW background did not lead to the formation of glomerular crescents. We have now generated a bicongenic strain, LEW.WCrgn1,2, in which WKY Crgn1 and Crgn2 are both introgressed into the LEW genetic background. These rats show development of NTN phenotypes, including glomerular crescents. Furthermore, we characterised macrophage function and glomerular cytokine profiles in this new strain. Additionally, we show that LEW.WCrgn1,2 rats are resistant to the development of glomerular crescents that is usually induced following immunisation with recombinant rat alpha 3(IV)NC1, the specific Goodpasture autoantigen located in the glomerular basement membrane against which the immune response is directed in experimental autoimmune glomerulonephritis. Our results show that the new bicongenic strain responds differently to two distinct experimental triggers of CRGN. This is the first time that CRGN has been induced on a normally resistant rat genetic background and identifies the LEW.WCrgn1,2 strain as a new, potentially valuable model of macrophage-dependent glomerulonephritis.

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:

Related Products

Related Resources

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

Inquiry Basket