Recombinant human C9 protein; Source: E. coli derived, PET30a; Tag: 6*His; Domain: 22-313 aa of BC020721
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:1000-1:8000
General Notes
This product (Catalog No. DPABH-00899) is a rabbit-derived polyclonal antibody raised against human C9, targeting the amino acid region 22–313 of the protein. It has been affinity purified by Protein A. The final consequence of complement activation is the formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. The MAC plays a critical role in complement-mediated hemolysis and inflammasome activation (PMID: 27444648). Complement component 9 (C9) is 1 of 5 late-acting proteins of complement that participate in the assembly and function of the MAC. C9 may be cleaved by thrombin to generate fragments C9a (25 kDa) and C9b (37 kDa) (PMID: 7523406). DPABH-00899 is suitable for use in Western blot (WB) (1:1,000–1:8,000) application. DPABH-00899 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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