Anti-C8G monoclonal antibody (DCABH-7160)

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
FQS22385
Species Reactivity
Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C8G aa 150 to the C-terminus. The exact sequence is proprietary.Database link: P07360
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/1000 - 1/2000; ICC/IF: 1/50 - 1/100;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
C8G; complement component 8, gamma polypeptide; complement component C8 gamma chain; C8C; MGC142186
Entrez Gene ID
UniProt ID

Product Background


Gene summary
C8G (Complement Component 8, Gamma Polypeptide) is a Protein Coding gene. Diseases associated with C8G include immunodeficiency due to a late component of complement deficiency and acute salpingo-oophoritis. Among its related pathways are Complement and coagulation cascades and Immune System. GO annotations related to this gene include protein complex binding and complement binding. The protein encoded by this gene belongs to the lipocalin family. It is one of the three subunits that constitutes complement component 8 (C8), which is composed of a disulfide-linked C8 alpha-gamma heterodimer and a non-covalently associated C8 beta chain. C8 participates in the formation of the membrane attack complex (MAC) on bacterial cell membranes. While subunits alpha and beta play a role in complement-mediated bacterial killing, the gamma subunit is not required for the bactericidal activity.
Antigen Description
C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol. The function about C8G antigen include binding; complement binding; retinol binding; transporter activity.
Pathway
Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem.

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References


Hepatotoxicity and mechanism study of chrysophanol-8-O-glucoside in vitro

BIOMEDICINE & PHARMACOTHERAPY

Authors: Lin, Longfei; Yuan, Fang; Liu, Yuling; Zhong, Ming; Xie, Tanggui; Ni, Jian; Li, Hui

To better understand the hepatotoxicity of anthraquinone glycosides, the hepatotoxicity of six anthraquinone glycosides was evaluated. The results show that chrysophanol-8-O-glucoside(C8G) has strong hepatotoxicity and can lead to increased LDH leakage and ROS, decreased GSH and MMP in L-02 hepatocytes. The results of C8G hepatotoxicity proteomics shows that, a total of 773 differentially expressed proteins were screened and analyzed using GO analysis and Pathway enrichment analysis. Our results show that C8G can lead to abnormal oxidative phosphorylation by inhibiting the function of mitochondrial complexes, resulting in decreased mitochondrial membrane potential (MMP), increased reactive oxygen species (ROS), and eventually resulting in mitochondrial damage and apoptosis. Western blot results verified the accuracy of quantitative proteomic results, and also evaluated the expression of Bax, caspase-3, -8, -9, Bcl-2, Cyt C in the mitochondria and cytosolic. The mitochondrial respiratory chain complexes activity assay result also confirmed that C8G could inhibit the activity of all mitochondrial complexes. The results of this study indicate that the hepatotoxicity mechanism of C8G is related to mitochondrial dysfunction, especially the mitochondrial complex function.

Coding polymorphisms in the genes of the alternative complement pathway and abdominal aortic aneurysm

INTERNATIONAL JOURNAL OF IMMUNOGENETICS

Authors: Bradley, D. T.; Badger, S. A.; Bown, M. J.; Sayers, R. D.; Hughes, A. E.

P>Variants in the genes of the alternative complement pathway are associated with risk of numerous inflammatory diseases. Abdominal aortic aneurysm is associated with inflammation and is a common cause of illness and death among European populations. This study tested 49 single nucleotide polymorphisms, including common putatively functional polymorphisms, in the genes of the alternative complement cascade (CFH, CFB, CFD, CFI, properdin, CR1, CR1L, CR2, CD46, vitronectin, C3, C5, C6, C7, C8A, C8B, C8G and C9). The study group were 434 cases with infra-renal aortic diameter >= 30 mm and 378 disease-free controls from two UK centres, all with self-reported European ancestry. There was no evidence for significant association with presence or size of aneurysm following correction for multiple testing. This study suggests that variation in the genes of the alternative pathway is not an important cause of abdominal aortic aneurysm development.

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