Human Anti-Blood Group A Antigen Hybridoma [IBB2] (CSC-H0827)

This hybridoma produces mAbs against blood Group A antigen Datasheet

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General Information

Fusion Species
Human X Human Hybridoma
Immunological Donor
Cell Line Description
Splenic B cells from a patient were fused with LTR228 lymphoblastoid cells.
Growth Properties

Culture Method

Complete Growth Medium
IMDM, supplemented with 15% FBS
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Liquid nitrogen vapor phase.

Freezing medium: to complete growth medium, add 10%(v/v) DMSO


The antigens which determine blood types belong to glycoproteins and glycolipids. There are three types of blood-group antigens: O, A, and B. They differ only slightly in the composition of carbohydrates.


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