Human Anti-Blood Group A Antigen Hybridoma [IBB2] (CSC-H0827)

This hybridoma produces mAbs against blood Group A antigen Datasheet

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General Information


Fusion Species
Human X Human Hybridoma
Immunological Donor
Human
Clone
IBB2
Cell Line Description
Splenic B cells from a patient were fused with LTR228 lymphoblastoid cells.
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
IMDM, supplemented with 15% FBS
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 10%(v/v) DMSO

Target


Introduction
The antigens which determine blood types belong to glycoproteins and glycolipids. There are three types of blood-group antigens: O, A, and B. They differ only slightly in the composition of carbohydrates.

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