Anti-Bacteria Cap32-34 Monoclonal antibody (DMAB9418)
Mouse Anti-Bacteria Cap32-34 Monoclonal antibody for WB, IHC
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Cap32/34 from Dictyostelium discoideum.
The heterodimeric F-actin capping protein cap32/34 from Dictyostelium discoideum is a typical member of a widely distributed family of cytoskeletal proteins. To analyze its regulation and structure/function relationships we cloned and expressed the subunits separately in Escherichia coli using the ATG-expression vector pT7-7. Studies on the viscosity of F-actin solutions and the kinetics of actin polymerization in the presence of single subunits or the reconstituted protein showed that capping of F-actin absolutely requires the heterodimeric conformation. This activity can be inhibited by phosphatidyl bisphosphate (PIP2), an important component in signal transduction. The regulation of cap32/34 by PIP2 suggests an involvement of this protein in the re-organization of the actin cytoskeleton upon stimulation of D. discoideum cells with chemoattractant.
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HARTMANN, H; SCHLEICHER, M; et al. HETERODIMERIC CAPPING PROTEINS CONSTITUTE A HIGHLY CONSERVED GROUP OF ACTIN-BINDING PROTEINS. DEVELOPMENTAL GENETICS 11:369-376(1990).
JUNGBLUTH, A; VONARNIM, V; et al. STRONG INCREASE IN THE TYROSINE PHOSPHORYLATION OF ACTIN UPON INHIBITION OF OXIDATIVE-PHOSPHORYLATION - CORRELATION WITH REVERSIBLE REARRANGEMENTS IN THE ACTIN SKELETON OF DICTYOSTELIUM CELLS. JOURNAL OF CELL SCIENCE 107:117-125(1994).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.