Anti-Bacteria Cap32-34 Monoclonal antibody (DMAB9418)

Mouse Anti-Bacteria Cap32-34 Monoclonal antibody for WB, IHC Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Species Reactivity
Bacteria
Immunogen
Cap32/34 from Dictyostelium discoideum.
Conjugate
Unconjugated

Target


Alternative Names
Cap32/34

Product Background


Antigen Description
The heterodimeric F-actin capping protein cap32/34 from Dictyostelium discoideum is a typical member of a widely distributed family of cytoskeletal proteins. To analyze its regulation and structure/function relationships we cloned and expressed the subunits separately in Escherichia coli using the ATG-expression vector pT7-7. Studies on the viscosity of F-actin solutions and the kinetics of actin polymerization in the presence of single subunits or the reconstituted protein showed that capping of F-actin absolutely requires the heterodimeric conformation. This activity can be inhibited by phosphatidyl bisphosphate (PIP2), an important component in signal transduction. The regulation of cap32/34 by PIP2 suggests an involvement of this protein in the re-organization of the actin cytoskeleton upon stimulation of D. discoideum cells with chemoattractant.

Citations


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References


HARTMANN, H; SCHLEICHER, M; et al. HETERODIMERIC CAPPING PROTEINS CONSTITUTE A HIGHLY CONSERVED GROUP OF ACTIN-BINDING PROTEINS. DEVELOPMENTAL GENETICS 11:369-376(1990).
JUNGBLUTH, A; VONARNIM, V; et al. STRONG INCREASE IN THE TYROSINE PHOSPHORYLATION OF ACTIN UPON INHIBITION OF OXIDATIVE-PHOSPHORYLATION - CORRELATION WITH REVERSIBLE REARRANGEMENTS IN THE ACTIN SKELETON OF DICTYOSTELIUM CELLS. JOURNAL OF CELL SCIENCE 107:117-125(1994).

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