Anti-BVDV Monoclonal antibody

Name: Anti-BVDV Monoclonal antibody
SKU: DMAB28412
Rating: 5 (1 reviews)

Mouse Anti-BVDV Monoclonal antibody for IF, IHC

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COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG2a

Species Reactivity

BVDV

Conjugate

Unconjugated

Target

Alternative Names

Bovine Viral Diarrhea Virus; Mouse Anti-BVDV Monoclonal Antibody; Anti-BVDV Monoclonal Antibody; BVDV Monoclonal Antibody Mouse Anti-BVDV MAb; Anti-BVDV MAb; BVDV MAb; Mouse Anti-BVDV Antibody; Anti-BVDV Antibody; BVDV Antibody

Citations

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Cell-to-Cell Transmission Is the Main Mechanism Supporting Bovine Viral Diarrhea Virus Spread in Cell Culture

Merwaiss F, Czibener C, Alvarez DE

J Virol2019 JanPubMed ID: 30404802Read Article

Applications: Neutralization
Reactive species: Bovine
"Abstract: After initiation of an infective cycle, spread of virus infection can occur in two fundamentally different ways: (i) viral particles can be released into the external environment and diffuse through the extracellular space until they interact with a new host cell, and (ii) virions can remain associated with infected cells, promoting the direct passage between infected and uninfected cells that is referred to as direct cell-to-cell transmission. Although evidence of cell-associated transmission has accumulated for many different viruses, the ability of members of the genus Pestivirus to use this mode of transmission has not been reported. In the present study, we used a novel recombinant virus expressing the envelope glycoprotein E2 fused to mCherry fluorescent protein to monitor the spreading of bovine viral diarrhea virus (BVDV) (the type member of the pestiviruses) infection. To demonstrate direct cell-to-cell transmission of BVDV, we developed a cell coculture system that allowed us to prove direct transmission from infected to uninfected cells in the presence of neutralizing antibodies."
Article snippet: Neutralizing titers were estimated by cytopathic effect reduction assays with the neutralizing serum, the purified IgG fraction, and an anti-BVDV MAb (DMAB28412; Creative Diagnostics) raised against E2.

Collect supernatants of transfected cells and infect a new monolayer of MDBK cells to assess the production of infectious viruses. (Merwaiss, <i>et al</i>, 2019)

Figure 1. Time course of infection of MDBK cells with cytopathic and noncytopathic BVDV/mCherry-E2.

The recombinant protein reduced BVDV cytopathic effect in a dose-dependent manner, reaching total inhibition at 1 µg/ml. (Merwaiss F, <i>et al</i>, 2019)

Figure 2. Functionality of recombinant E2 tested in a cytopathic effect reduction assay. (Merwaiss, Fernando, Cecilia Czibener, and Diego E. Alvarez, 2019)

Experimental infection of mice with noncytopathic bovine viral diarrhea virus 2 increases the number of megakaryocytes in bone marrow

Lee KH, Han DG, Kim S, Choi EJ, Choi KS

Virol J2018 JulPubMed ID: 30055639Read Article

Applications: IHC
Reactive species: BVDV
"Abstract: Background: Bovine viral diarrhea virus (BVDV) causes significant economic losses worldwide in the cattle industry through decrease in productive performance and immunosuppression of animals in herds. Recent studies conducted by our group showed that mice can be infected with BVDV-1 by the oral route. The purpose of this study was to assess the clinical signs, hematological changes, histopathological lesions in lymphoid tissues, and the distribution of the viral antigen after oral inoculation with a Korean noncytopathic (ncp) BVDV-2 field isolate in mice. Methods: Mice were orally administered a low or high dose of BVDV-2; blood and tissue samples were collected on days 2, 5, and 9 postinfection (pi). We monitored clinical signs, hematological changes, histopathological lesions, and tissue distribution of a viral antigen by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) and then compared these parameters with those in ncp BVDV-1 infections."
Article snippet: For BVDV antigen detection, 5-μm-thick paraffin-embedded tissue sections were deparaffinized and hydrated through a graded alcohol series before heat-induced antigen retrieval in 10 mM sodium citrate buffer (pH 6) for 30 min. A primary anti-BVDV monoclonal antibody (DMAB28412; Creative Diagnostics, Shirley, NY, USA) was used according to the manufacturer’s instructions.

IHC for viral antigens in mice orally inoculated with a low dose of ncp BVDV-2 or mock-infected mice at day 5 postinfection.(Lee KH, <i>et al</i>, 2018)

Figure 1. IHC of the viral antigen on day 5 pi in mice inoculated orally with a low (4 × 10⁵ TCID₅₀) of ncp BVDV-2 or in mice with the mock infection.

Experimental infection with non-cytopathic bovine viral diarrhea virus 1 in mice induces inflammatory cell infiltration in the spleen

Yu-Jung Han, Young-Je Kwon, Kyung-Hyun Lee, Eun-Jin Choi, Kyoung-Seong Choi

Archives of Virology2016 JulPubMed ID: 27376375Read Article

Applications: IHC
Reactive species: BVDV
"Abstract: Previously, our study showed that oral inoculation of mice with cytopathic (cp) bovine viral diarrhea virus (BVDV) led to lymphocyte depletion and increased numbers of megakaryocytes in the spleen as well as thrombocytopenia and lymphopenia. In the present study, to investigate the possible differences in the detection of viral antigen, histopathological lesions, and hematologic changes between non-cytopathic (ncp) BVDV1 and cp BVDV1, mice were orally administered low and high doses of ncp BVDV1 and were necropsied at days 0, 2, 5, and 9 postinfection (pi). None of the ncp BVDV1-infected mice exhibited clinical signs of illness, unlike those infected with cp BVDV1. Statistically significant thrombocytopenia was observed during ncp BVDV1 infection, and lymphopenia was found only in mice infected with a high dose at day 9 pi. Interestingly, ncp BVDV1 infection increased the numbers of basophils, eosinophils, neutrophils, and monocytes in some infected mice. Viral antigen was detected in the lymphocytes of the spleen, mesenteric lymph nodes, Peyer’s patches, and bone marrow by immunohistochemistry. Lymphoid depletion was evident in the mesenteric lymph nodes of mice infected with a high dose and also found in the Peyer’s patches of some infected mice. Infiltration of inflammatory cells, including neutrophils and monocytes, and an increased number of megakaryocytes were seen in the spleen. These results suggest that the distribution of viral antigens is not associated with the presence of histopathological lesions. Inflammatory cell infiltration was observed in the spleens as a result of viral replication and may be attributable to the host reaction to ncp BVDV1 infection. Together, these findings support the possibility that mice can be used as an animal model for BVDV infection."
Article snippet: For BVDV antigen detection, IHC staining was performed on formalin-fixed paraffin-embedded blocks of harvested tissue samples, including the kidney, liver, lung, spleen, mesenteric lymph nodes, intestines, stomach, GALT, BALT, and femur as described previously. The primary anti-BVDV monoclonal antibody (DMAB28412; Creative Diagnostics, Shirley, NY, USA) was used according to the manufacturer’s instructions.

Immunohistochemistry of viral antigen at day 5 pi after oral inoculation with ncp BVDV1. (Yu-Jung Han, <i>et al</i>, 2016)

Figure 1. Immunohistochemistry of viral antigen at day 5 pi after oral inoculation with ncp BVDV1.

Primary Cells from a CD46-Edited Bovine Heifer Have Reduced BVDV Susceptibility Despite Viral Adaptation to Heparan Sulfate

Krueger AC, Vander Ley BL, Heaton MP, Sonstegard TS, Workman AM

Viruses2025 AprPubMed ID: 40431646Read Article

Applications: FC
Reactive species: BVDV
"Abstract: A precision genome edit in the bovine CD46 gene (A82LPTFS87) dramatically reduced bovine viral diarrhea virus (BVDV) susceptibility in a cloned heifer. However, pathogen evolution threatens the long-term efficacy of such interventions. Here, our aim is two-fold: first, to determine whether BVDV can adapt in vitro to use the edited CD46 receptor to infect Madin-Darby bovine kidney (MDBK) cells, and second, to evaluate the ex vivo infectivity of culture-adapted viruses in cells from the CD46-edited heifer. Serial passage of BVDV on CD46-edited MDBK cells selected for virus variants capable of CD46-independent infection. Virus genome sequencing revealed mutations in the viral ERNS gene predicted to enhance HS-mediated entry. HS adaptation was confirmed by inhibiting virus infection with heparin or Heparinase I/III treatment. A naturally occurring HS-adapted field isolate from a persistently infected calf showed similar results. However, when tested on primary cells from the CD46-edited heifer, HS-adapted viruses showed reduced infectivity in skin fibroblasts, monocytes, and lymphocytes in a manner that correlated with HS expression. Thus, although BVDV can adapt to use HS as an alternative entry receptor, HS adaptation does not overcome the protection conferred by the CD46 edit in all relevant cell types."
Article snippet: Cells were pelleted and resuspended in 100 µL of an antibody dilution buffer (PBS containing 1% BSA and 0.1% w/v saponin) containing a 1:200 dilution of an anti-BVDV monoclonal antibody (VMRD, Catalog no. 348. Pullman, WA, USA) used for MDBK cells or (DMAB, Catalog no. 28412. Creative Diagnostics, Shirley, NY, USA) used for lymphocytes and incubated for 30 min at room temperature.

Assessment of BVDV infection in primary lymphocytes via flow cytometry. (Krueger AC, <i>et al</i>, 2025)

Figure 1. Assessment of BVDV infection in primary lymphocytes via flow cytometry.

Documents

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QA & Reviews

Q & A

Ask a Question

Q: Which isotype does this antibody recognize?

A: This antibody can be used to detect most strains of BVDV and will detect both Type 1 and Type 2.

Q: Does this antibody bind BVDV type 1 and type 2 all together?

A: This antibody should react with most BVDV strains, but was found to not interact with the Oregon C24V strain.

Customer Reviews

References

Serologic evidence of HoBi-like virus circulation in Argentinean water buffalo

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION

Authors: Pecora, Andrea; Perez Aguirreburualde, Maria S.; Malacari, Dario A.; Zabal, Osvaldo; Sala, Juan M.; Konrad, Jose L.; Caspe, Sergio G.; Bauermann, Fernando; Ridpath, Julia; Dus Santos, Maria J.

Abstract

HoBi-like pestiviruses (also known as bovine viral diarrhea virus 3) have been sporadically reported from naturally infected cattle in Brazil, Asia, and Europe. Although HoBi-like viruses seem to be endemic in Brazilian cattle and buffalo, they have not been studied in the other countries of South America to our knowledge. Herein we report serologic results of buffalo from 12 large farms in Argentina located near the Brazilian border. These buffalo were not vaccinated against pestiviruses. Our results indicate that HoBi-like virus may be circulating in the northeastern region of Argentina given that half of the analyzed animals showed high levels of neutralizing antibodies against the pestivirus. The HoBi-like seropositive animals were also checked for neutralizing antibodies against BVDV-1a, BVDV-1b, and BVDV-2, and in most cases these animals had low levels or no detectable antibodies against these other pestiviruses. Our study suggests a need for continued pestivirus surveillance in Argentinean cattle and buffalo.

A direct high-throughput in Cell-ELISA for measuring infectivity of cytopathic and non-cytopathic bovine viral diarrhoea virus strains applied to the assessment of antiviral activity

JOURNAL OF VIROLOGICAL METHODS

Authors: Eugenia Quintana, Maria; Barone, Lucas; Belen Forlenza, Maria; Vanesa Trotta, Myrian; Turco, Cecilia; Celeste Mansilla, Florencia; Patricia Cardoso, Nancy; Victoria Capozzo, Alejandra

Abstract

Low-cost high-throughput methods applicable to any virus strain are required for screening antiviral compounds against multiple field strains. Colorimetric cell-viability assays are used for this purpose as long as the viruses are cytopathic (CP) in cell culture. However, bovine viral diarrhoea virus (BVDV) strains circulating in the field are mostly non-cytopathic (NCP). An In Cell-ELISA aimed to measure viral infectivity by detecting a conserved protein produced during viral replication (non-structural protein 3, "NS3") was developed. The ELISA is performed without harvesting the cells, directly on the 96-wells culture plate. NS3 In Cell-ELISA was tested for its ability to assess BVDV-specific antiviral activity of recombinant bovine type I and III IFNs. Results correlated to those measured by qRT-PCR and virus titration. NS3 In Cell-ELISA was also efficient in estimating the IC50 of two compounds with different antiviral activity. Estimation of the 50% inhibition dose of each IFN using six BVDV strains of different biotype and genotype showed that CP strains were more susceptible to both IFNs than NCP, while type 2 NCP viruses were more sensitive to IFN-I. The In Cell-ELISA format using a detector antibody against a conserved non-structural protein can be potentially applied to accurately measure infectivity of any viral strain.