GeneralInformation
Fusion Species
Bovine X Mouse Heterohybridoma
Immunological Donor
Bovine
Cell Line Description
Animals were immunized with live BHV-1. Lymph node cells were fused with Sp2/0 mouse myeloma cells. Initial hybrids were refused with additional lymph node cells from the same animal and reselected. The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
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Lazic, S; Perovic, G; et al. Examination of the relationship between net extinction in ELISA and virus neutralization methods for estimating the titer of neutralizing antibodies against bovine herpesvirus 1.. ACTA VETERINARIA-BEOGRAD 48:99-104(1998).
COX, GJM; ZAMB, TJ; et al. BOVINE HERPESVIRUS-1 - IMMUNE-RESPONSES IN MICE AND CATTLE INJECTED WITH PLASMID DNA. JOURNAL OF VIROLOGY 67:5664-5667(1993).
COA
Certificate of Analysis
