Bovine Anti-Virus BHV-1 Heterohybridoma [Alpha CM6D3.98] (CSC-H0899)

This hybridoma produces mAbs (IgG1) against virus BHV-1

General Information


Immunogen
Live BHV-1
Isotype
IgG1
Fusion Species
Bovine X Mouse Heterohybridoma
Immunological Donor
Bovine
Myeloma
Sp2/0
Clone
alpha CM6D3.98
Cell Line Description
Animals were immunized with live BHV-1. Lymph node cells were fused with Sp2/0 mouse myeloma cells. Initial hybrids were refused with additional lymph node cells from the same animal and reselected. The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
DMEM with 4.5 g/L glucose, supplemented with 10% horse serum
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO

Target


Introduction
Bovine herpesvirus 1 (BoHV-1) is a virus of the family Herpesviridae and the subfamily Alphaherpesviridae, known to cause several diseases worldwide in cattle, including rhinotracheitis, vaginitis, balanoposthitis, abortion, conjunctivitis, and enteritis. BoHV-1 is also a contributing factor in shipping fever, also known as Bovine Respiratory Disease (BRD). It is spread horizontally through sexual contact, artificial insemination, and aerosol transmission and it may also be transmitted vertically across the placenta. BoHV-1 can cause both clinical and subclinical infections, depending on the virulence of the strain. Although these symptoms are mainly non-life threatening it is an economically important disease as infection may cause a drop in production and affect trade restrictions. Like other herpesviruses, BoHV-1 causes a lifelong latent infection and sporadic shedding of the virus. The sciatic nerve and trigeminal nerve are the sites of latency. A reactivated latent carrier is normally the source of infection in a herd. The clinical signs displayed are dependent on the virulence of the strain. There is a vaccine available which reduces the severity and incidence of disease. Some countries in Europe have successfully eradicated the disease by applying a strict culling policy.

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References


Meyer, G; Bare, O; et al. Identification and characterization of bovine herpesvirus type 5 glycoprotein H gene and gene products. JOURNAL OF GENERAL VIROLOGY 80:2849-2859(1999).
Joseph, T; Lyaku, J; et al. Use of epitope mapping to identify a PCR template for protein amplification and detection by enzyme-linked immunosorbent assay of bovine herpesvirus type 1 glycoprotein D. JOURNAL OF CLINICAL MICROBIOLOGY 40:4045-4050(2002).

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