GeneralInformation
Immunogen
Irradiated 5C2 mouse B cell lymphoma cells
Fusion Species
Rat X Mouse Heterohybridoma
Immunological Donor
Rat Popliteal lymph node
Cell Line Description
Animals were immunized in the hind footpad with irradiated 5C2 mouse B cell lymphoma cells that had been cultured in the presence of dibutyryl-cAMP. Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells. The antibody will neutralize the B7.2 antigen, but does not react with the B7.1 antigen.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 0.5-1x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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Neighbors, M; Apt, D; et al. EpCAM-specific vaccine response by modified antigen and chimeric costimulatory molecule in cynomolgus monkeys. JOURNAL OF IMMUNOTHERAPY 31:644-655(2008).
Samuelsen, M; Nygaard, UC; et al. Allergy adjuvant effect of particles from wood smoke and road traffic. TOXICOLOGY 246:124-131(2008).
COA
Certificate of Analysis
