Anti-Annexin V IgG/IgM ELISA Kit

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Anti-Annexin V IgG/IgM is an ELISA test system for the quantitative measurement of IgG and IgM class autoantibodies against Annexin V in human serum or plasma. This product is intended for research use only. Not for diagnostic procedures.

Contents of Kit

1.Microplate, 1, One divisible microplate consisting of 12 modules of 8 wells each. Ready to use. Product code on module: ANX
2.Calibrator A 0 U/ml, 1.5ml, containing serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
3.Calibrator B 6.3 U/ml, 1.5ml, containing annexin V antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
4.Calibrator C 12.5 U/ml, 1.5ml, containing annexin V antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
5.Calibrator D 25 U/ml, 1.5ml, containing annexin V antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
6.Calibrator E 50 U/ml, 1.5ml, containing annexin V antibodies in a serum/buffer matrix (PBS, BSA, NaN₃ 0.09%), yellow. Ready to use.
7.Calibrator F 100 U/ml, 1.5ml, containing annexin V antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
8.Control positive IgG, 1.5ml, containing annexin V antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
9.Control positive IgM, containing annexin V antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
10.Control negative, 1.5ml, containing annexin V antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
11.Sample Buffer P, 20ml, containing PBS, BSA, detergent, preservative sodium azide 0.09%, yellow, concentrate (5 x).
12.Enzyme Conjugate; 15ml, containing anti-human IgG antibodies, HRP labelled; PBS, BSA, detergent, preservative PROCLIN 0.05%, light red. Ready to use.
13.Enzyme Conjugate; 15ml, containing anti-human IgM antibodies, HRP labelled; PBS, BSA, detergent, preservative PROCLIN 0.05%, light red. Ready to use.
14.TMB Substrate; 15ml, containing 3,3', 5,5'- Tetramethylbenzidin, colorless. Ready to use.
15.Stop solution; 15ml, contains acid. Ready to use.
16.Wash Buffer, 20ml, containing Tris, detergent, preservative sodium azide 0.09%; 50 x conc.
17.Certificate of Analysis, 1

Storage

1.Store test kit at 2-8°C in the dark.
2.Do not expose reagents to heat, sun, or strong light during storage and usage.
3.Store microplate sealed and dessicated in the clip bag provided.
4.Shelf life of the unopended test kit is 18 months from day of production. Unopened reagents are stable until expiration of the kit. See labels for individual batch.
5.Diluted Wash Buffer and Sample Buffer are stable for at least 30 days when stored at 2-8°C. We recommend consumption on the same day.

Performance Characteristics

Calibration
This assay system is calibrated in relative arbitrary units, since no international reference preparation is available for this assay.
Detection Range
The calculation range of this ELISA assay is IgG: 0 - 100 U/ml, IgM: 0 - 100 U/ml