Anti-ATP5B monoclonal antibody (DCABH-5523)

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
KN21-01
Species Reactivity
Human, Mouse, Rat, Zebrafish
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:100-1:500 IHC: 1:50-1:200; IP: 1:10-1:50
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
ATP 5B; ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide; ATP synthase subunit beta mitochondrial; ATP synthase subunit beta, mitochondrial; atp5b; ATPB
Entrez Gene ID

Citations


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References


ATP synthase beta-subunit abnormality in pancreas islets of rats with polycystic ovary syndrome and type 2 diabetes mellitus

JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES

Authors: Li, Wei; Li, Sai-jiao; Yin, Tai-lang; Yang, Jing; Cheng, Yan

This study investigated the abnormal expression of ATP synthase beta-subunit (ATPsyn-beta) in pancreas islets of rat model of polycystic ovary syndrome (PCOS) with type 2 diabetes mellitus (T2DM), and the secretion function changes after up-regulation of ATP5b. Sixty female SD rats were divided into three groups randomly and equally. The rat model of PCOS with T2DM was established by free access to the high-carbohydrate/high-fat diet, subcutaneous injections of DHEA, and a single injection of streptozotocin. The pancreas was removed for the detection of the ATPsyn-beta expression by immunohistochemical staining, Western blotting and reverse transcription-PCR (RT-PCR). The pancreas islets of the rats were cultured, isolated with collagenase V and purified by gradient centrifugation, and the insulin secretion after treatment with different glucose concentrations was tested. Lentivirus ATP5b was successfully constructed with the vector of GV208 and transfected into the pancreas islets for the over-expression of ATPsyn-beta. The insulin secretion and intracellular ATP content were determined after transfection of the PCOS-T2DM pancreas islets with Lenti-ATP5b. The results showed that the expression of ATPsyn-beta protein and mRNA was significantly decreased in the pancreas of PCOS-T2DM rats. The ATP content in the pancreas islets was greatly increased and the insulin secretion was improved after the up-regulation of ATPsyn-beta in the pancreas islets transfected with lenti-ATP5b. These results indicated that for PCOS, the ATPsyn-beta might be one of the key factors for the attack of T2DM.

ATP5b and beta 2-microglobulin are predictive markers for the prognosis of patients with gallbladder cancer

JOURNAL OF MOLECULAR HISTOLOGY

Authors: Sun, Jianning; Yang, Zhu-lin; Miao, Xiongying; Zou, Qiong; Li, Jinghe; Liang, Lufeng; Zeng, Guixiang; Chen, Senlin

The differences in clinical, pathological, and biological characteristics between adenocarcinoma (AC) and squamous cell/adenosquamous carcinoma (SC/ASC) of the gallbladder have not been well documented. This study investigates the clinical and pathological associations of ATP5B and beta 2M with benign and malignant lesions of the gallbladder. In this study, ATP5B and beta 2M expression in 46 SC/ASCs and 80 ACs were examined using immunohistochemistry. The rate of ATP5B positive expression was significantly lower, while the rate of beta 2M expression was significantly higher, in AC and SC/ASC than in gallbladder adenomas, gallbladder polyps, or gallbladder epithelium with stone (P < 0.01). More SC/ASCs had larger tumor mass and good differentiation compared to ACs. Positive beta 2M and negative ATP5B expression were significantly associated with large tumor size, high TNM stage, lymph node metastasis, and invasion of SC/ASCs and ACs. Univariate Kaplan-Meier analysis showed that positive beta 2M (P < 0.05 or P < 0.001) expression and negative ATP5B (P < 0.001) expression were significantly associated with decreased overall survival in both SC/ASC and AC patients. Multivariate Cox regression analysis showed that negative ATP5B expression is an independent-prognostic factor for poor prognosis in both SC/ASC (P < 0.01) and AC (P < 0.001) patients. Positive beta 2M expression is an independent-prognostic factor for poor prognosis in AC (P < 0.05) patients. Our study suggested that positive beta 2M expression or loss of ATP5B expression in tumor tissues is closely related to the metastasis, invasion, and poor-prognosis of gallbladder cancer.

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