Anti-4-amino Biphenyl DNA monoclonal antibody (DMAB15630)

Mouse anti-4-amino Biphenyl DNA monoclonal antibody for IHC, ICC, IF, ELISA Datasheet

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Specifications


Host Species
Mouse
Clone
5D22
Species Reactivity
N/A
Immunogen
4-amino Biphenyl.
Conjugate
Unconjugated

Applications


Application Notes
Immunohistochemistry (1:50-1:100); Immunocytochemistry (1:50-1:100) & The optimal working dilution should be determined by the end user.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
4-AMBP DNA, 4-Aminobiphenyl DNA; Mouse Anti-4-amino Biphenyl DNA Monoclonal Antibody; Anti-4-amino Biphenyl DNA Monoclonal Antibody; 4-amino Biphenyl DNA Monoclonal Antibody Mouse Anti-4-amino Biphenyl DNA MAb; Anti-4-amino Biphenyl DNA MAb; 4-amino Biphe

Product Background


Antigen Description
4-amino Biphenyl (4-ABP) is an aromatic amine (arylamine). 4-ABP can be found as a constituent of tobacco smoke, in the form of an environmental carcinogen. It is metabolized by hepatic cytochrome P4501A2 to yield a direct-acting mutagen known as N-hydroxy-aminobiphenyl. Yet, the accumulation of biologically active metabolites is dependent on the activation and detoxification of N-acetylation. 4-ABP has been shown to be a bladder carcinogen in humans and rodents. As well as genetic factors, 4-ABP may also play a role in breast cancer. It is hypothesized that smokers may have higher levels of DNA adducts than non-smokers and that the role of 4-ABP-DNA adducts and their relationship to tobacco exposure may be related to breast tumorigenesis. The antibody has been used to investigate the role of 4-ABP in the study of human breast cancer by measuring 4-ABP-DNA adducts. Cellular Localization: NuclearApplications: Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution:ELISA: 1:50-1:100Immunohistochemistry: 1:50-1:100Immunocytochemistry: 1:50-1:100Immunofluorescence: 1:50-1:100Optimal dilutions to be determined by the researcher. Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Citations


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References


GROOPMAN, JD; DONAHUE, PR; et al. POLYCLONAL AND MONOCLONAL-ANTIBODIES RECOGNIZING 4-AMINO-BIPHENYL (4ABP) AND ITS DNA ADDUCTS. PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH 26:87-87(1985).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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