Serum, EDTA, Heparin Plasma, Urine, Fecal Extracts, Tissue Culture Media
The Aldosterone Immunoassay kit is designed to quantitatively measure Aldosterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total aldosterone in extracted serum or plasma and fecal samples.
Contents of Kit
1. Coated Clear 96 Well Plate
2. Aldosterone Standard
3. Aldosterone Antibody
4. Aldosterone Conjugate
5. Assay Buffer Concentrate
6. Wash Buffer Concentrate
7. TMB Substrate
8. Stop Solution
9. Plate Sealer
Sensitivity and Limit of Detection
Sensitivity was calculated by comparing the OD's for twenty wells run for each of the B0 and standard #6. The detection limit was determined at two (2) standard deviations from the B0 alon
Aldosterone, C21H28O5, is a mineralocorticoid first isolated by the husband and wife team of Simpson and Tait at University College, London in 1953. Initially called electrocortin, 21 mg was isolated from 500 kg of beef adrenal glands. Aldosterone controls the sodium-potassium balance through the unidirectional salt reabsorption in a variety of tissues and glands. Synthesized from cholesterol in the zona glomerulosa of the adrenal cortex, secretion is regulated through the renin-angiotensin system. Angiotensin II and potassium stimulate primary secretion by increasing the rate of production of the steroid. Peripheral aldosterone levels are dependant on age and body position and in a normal upright adult aldosterone levels are typically less than 300 pg/mL. Aldosterone is typically secreted as the 18-glucuronide and the terahydro-3-glucuronide and this excretion is generally 2-20 µg/24 hour urine collection. Aldosterone measurement is useful in the investigation of primary aldosteronism (i.e., adrenal adenoma or carcinoma and adrenal cortical hyperplasia) and secondary aldosteronism (renovascular disease, salt depletion, potassium loading, cardiac failure with ascites, pregnancy, Bartter syndrome). The renin-angiotensin system is the primary regulator of the synthesis and secretion of aldosterone. Increased concentrations of potassium in the plasma may directly stimulate adrenal production of the hormone. Under physiologic conditions, pituitary adrenocorticotropic hormone is not a major factor in regulating aldosterone secretion.
Typical Standard Curve - Overnight Incubation
Typical Standard Curve - 2 Hour Incubation
Reconstitution And Storage
All components of this kit should be stored at 4°C until the expiration date of the kit.