Aldosterone Enzyme Immunoassay Kit (DEIABL242)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

Size
96T
Sample
Serum, EDTA, Heparin Plasma, Urine, Fecal Extracts, Tissue Culture Media
Species Reactivity
N/A
Intended Use
The Aldosterone Immunoassay kit is designed to quantitatively measure Aldosterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total aldosterone in extracted serum or plasma and fecal samples.
Contents of Kit
1. Coated Clear 96 Well Plate
2. Aldosterone Standard
3. Aldosterone Antibody
4. Aldosterone Conjugate
5. Assay Buffer Concentrate
6. Wash Buffer Concentrate
7. TMB Substrate
8. Stop Solution
9. Plate Sealer
Storage
4°C
Performance Characteristics
OVERNIGHT INCUBATION

Sensitivity and Limit of Detection
Sensitivity was calculated by comparing the OD's for twenty wells run for each of the B0 and standard #6. The detection limit was determined at two (2) standard deviations from the B0 alon
General Description
Aldosterone, C21H28O5, is a mineralocorticoid first isolated by the husband and wife team of Simpson and Tait at University College, London in 1953. Initially called electrocortin, 21 mg was isolated from 500 kg of beef adrenal glands. Aldosterone controls the sodium-potassium balance through the unidirectional salt reabsorption in a variety of tissues and glands. Synthesized from cholesterol in the zona glomerulosa of the adrenal cortex, secretion is regulated through the renin-angiotensin system. Angiotensin II and potassium stimulate primary secretion by increasing the rate of production of the steroid. Peripheral aldosterone levels are dependant on age and body position and in a normal upright adult aldosterone levels are typically less than 300 pg/mL. Aldosterone is typically secreted as the 18-glucuronide and the terahydro-3-glucuronide and this excretion is generally 2-20 µg/24 hour urine collection. Aldosterone measurement is useful in the investigation of primary aldosteronism (i.e., adrenal adenoma or carcinoma and adrenal cortical hyperplasia) and secondary aldosteronism (renovascular disease, salt depletion, potassium loading, cardiac failure with ascites, pregnancy, Bartter syndrome). The renin-angiotensin system is the primary regulator of the synthesis and secretion of aldosterone. Increased concentrations of potassium in the plasma may directly stimulate adrenal production of the hormone. Under physiologic conditions, pituitary adrenocorticotropic hormone is not a major factor in regulating aldosterone secretion.
Standard Curve
Typical Standard Curve - Overnight Incubation

Typical Standard Curve - 2 Hour Incubation
Reconstitution And Storage
All components of this kit should be stored at 4°C until the expiration date of the kit.

Citations


Have you cited DEIABL242 in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

Online Inquiry

Name:
Phone: *
E-mail Address: *
Service & Products Interested: *
Project Description:
Verification Code: * Please input "diagnostics" as verification code.

Online Inquiry

  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket