Adenovirus IgG ELISA Kit (DEIA2382)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Human serum or plasma (citrate, heparin)
Species Reactivity
Human
Intended Use
The Adenovirus IgG ELISA is intended for the qualitative determination of IgG class antibodies against Adenovirus in human serum or plasma (citrate, heparin).
Contents of Kit
1. SORB MT Microtiterplate : 12 break-apart 8-well snap-off strips coated with Adenovirus antigens; in resealable aluminium foil.
2. SAM DIL IgG Sample Dilution Buffer: 1 bottle containing 100 mL of phosphate buffer (10 mM) for sample dilution; pH 7.2 ± 0.2; coloured yellow; ready to use; white cap; ≤ 0.0015% (v/v) CMIT/ MIT (3:1).
3. STOP SOLN Stop Solution: 1 bottle containing 15 mL sulphuric acid, 0.2 mol/L; ready to use; red cap.
4. WASH SOLN 20x Washing Buffer (20x conc.): 1 bottle containing 50 mL of a 20-fold concentrated phosphate buffer (0.2 M), pH 7.2 ± 0.2, for washing the wells; white cap.
5. ENZ CONJ Conjugate: 1 bottle containing 20 mL of peroxidase labelled antibody to human IgG in phosphate buffer (10 mM); coloured blue; ready to use; black cap.
6. SUB TMB TMB Substrate Solution: 1 bottle containing 15 mL 3,3',5,5'-tetramethylbenzidine (TMB), < 0.1 %; ready to use; yellow cap.
7. CAL C Positive Control: 1 vial containing 2 mL control; coloured yellow; ready to use; red cap; ≤ 0.02% (v/v) MIT.
8. CAL B Cut-off Control: 1 vial containing 3 mL control; coloured yellow; ready to use; green cap; ≤ 0.02% (v/v) MIT.
9. CAL A Negative Control: 1 vial containing 2 mL control; coloured yellow; ready to use; blue cap; ≤ 0.0015% (v/v) CMIT/ MIT (3:1). Controls are calibrated in arbitrary units against internal quality control specimens, since no international standard reference is available for this assay.
10. 1 Cover foil
11.1 Instruction for use (IFU)
12.Plate layout
For potential hazardous substances please check the safety data sheet.
CMIT: 5-chloro-2-methyl-4-isothiazolin-3-one
MIT: 2-methyl-2H-isothiazol-3-one
Storage
Store the kit at 2-8 °C. The opened reagents are stable up to the expiry date stated on the label when stored at 2-8 °C.
Precision
Sensitivity
The diagnostic sensitivity is defined as the probability of the assay of scoring positive in the presence of the specific analyte. It is 100% (95% confidence interval: 95.2% - 100%).
General Description
Adenoviruses are double-stranded DNA viruses of about 70-90 nm lacking an envelope. The capsid contains 252 capsomeres and shows icosahedral symmetry. The capsomeres consist of hexons, pentons and fiberprotein trimers which are responsible for the induction of group- and type-specific antibodies.
For the first time adenoviruses were isolated in 1953 from tonsils and adenoid tissue by Rowe. More than 80 adenoviruses are known at present. 47 out of them are pathogenic for men. They cause several diseases of different organic systems, mainly eyes, pharynx, respiratory and gastrointestinal system. Adenovirus infections are common and frequent. Most infections appear during childhood. They pass of latently so that the virus can still be detected in tonsils after two years. It is excrete via saliva and faeces. Gate to body are mouth, nasal pharynx and conjunctiva of the eye. Most infections pass off without symptoms. Around 5 % of all coughs and sneezes of children are caused by adenoviruses. Epidemics may occur in populations crowded together, for example acute respiratory disease in military groups, pharyngoconjunctival fever in swimming pools, and epidemic keratoconjunctivitis in medical facilities. Infection of hospitals and swimming pools gratify special demands on hygienics.

Infection or presence of pathogen may be identified by:
• Cell culture
• PCR
• Serology: e.g. by ELISA
• Virus isolation

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References


Anthropometry and performance characteristics of recreational advanced to elite female rock climbers

JOURNAL OF SPORTS SCIENCES

Authors: Giles, David; Barnes, Kimberly; Taylor, Nicola; Chidley, Corinna; Chidley, Joel; Mitchell, James; Torr, Oliver; Gibson-Smith, Edward; Espana-Romero, Vanesa

Despite climbing's popularity and an increasing number of female participants, there are limited anthropometric and performance data for this population. This study compares the characteristics of 55 experienced female climbers, divided into three categories (lower [ADV-L] and higher advanced [ADV-H] and elite [ELT]) based on self-reported ability. Data on climbing experience, body dimensions, body composition, flexibility, lower and upper-body power and finger strength were assessed. ELT climbers differed significantly from the ADV groups in age (Mean Difference [MD] = 8.8-9.8 yrs; despite smaller differences in years climbing MD = 1.6-2.4 yrs), greater climbing and hours training per week (MD = 3.0-3.7 h & MD = 0.9-1.6 h, respectively), and greater upper-body power (MD = 12.9-16.6 cm) and finger strength (MD = 51.6-65.4 N). Linear regression analysis showed finger strength and upper body power to be associated with ability, particularly when adjusting for descriptive and anthropometric variables (finger strength R-2 = 53% and 45%; upper-body power R-2 = 60% and 39% for boulder and sport, respectively). The findings support the importance of finger strength and upper-body power; changes in female anthropometric data over the last decade provide insight into the changing nature of the sport.

Measurements of the sediment flocculation characteristics in the Three Gorges Reservoir, Yangtze River

RIVER RESEARCH AND APPLICATIONS

Authors: Li, Wenjie; Yu, Chengfeng; Yang, Shengfa; Yang, Yunping; Yang, Wei; Xiao, Yi

The flocculation of fine sediments in the Three Gorges Reservoir (TGR) was confirmed in previous studies, but the flocculation characteristics have yet to be fully clarified. In this study, field measurements were conducted in the TGR to investigate the sediment flocculation characteristics. First, the instantaneous flow velocity and sediment concentration were measured through Acoustic Doppler velocimeter and sediment sampling. Then, the effective settling velocity was calculated based on the sediment diffusion theory to deduce the floc size and flocculation degree. Finally, the influences of particle size, flow velocity, and sediment concentration on flocculation were analyzed. Results showed that flocculation occurred in more than half of the sediments in the TGR, and the maximum flocculation degree was between 10 and 30. Flocculation weakened as particle diameter increased, with the critical particle size being approximately 0.018 mm, meaning that flocculation was unlikely to occur when the particle size exceeded the critical value. As the flow velocity increased, the flocculation degree first increased and then decreased, with the critical flow velocity being approximately 0.7 m/s, but the critical flow velocity increased with an increase in sediment concentration and tended to be a constant. The flocculation degree also increased with increasing sediment concentration and tended to be constant when the sediment concentration exceeded approximately 0.5 kg/m(3). The results provide new information on the flocculation characteristics of the TGR and should be useful for understanding and simulating fine sediment transport in the TGR.

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