10 ml AddaVax provided as a ready-to-use sterile emulsion.
Storage and Sability
AddaVax is shipped at room temperature and should be stored at 4˚C.
AddaVax is stable for 6 months. DO NOT FREEZE.
AddaVax is based on nano-emulsification of 2 components:
- Sorbitan trioleate (0.5% w/v) in squalene oil (5% v/v)
- Tween 80 (0.5% w/v) in sodium citrate buffer (10 mM, pH 6.5)
The nano-emulsion is produced using a microfluidizer and filtered through a 0.22-μm filter to remove large droplets and sterilize the final product. The particle size is ~ 160 nm.
AddaVax is a squalene-based oil-in-water nano-emulsion. Squalene is an oil more readily metabolized than the paraffin oil used in Freund’s adjuvants. AddaVax promotes a significant increase in antibody titers with reportedly more balanced Th1/Th2 responses than those obtained with alum. AddaVax is believed to act through a depot effect, enhancement of antigen persistence at the injection site, recruitment and activation of antigen presenting cells, and direct stimulation of cytokines and chemokines production by macrophages and granulocytes.
Preparation of Antigen-AddaVax Mixture
Antigens are preferentially diluted in saline or phosphate buffers. The amount of protein or conjugated peptide used for the primary immunization can be adjusted depending upon availability and immunogenicity of the antigen. Mice can be injected subcutaneously (s.c.)
with 1 to 10 μg of endotoxin-free ovalbumin.
1. Bring AddaVax to room temperature.
2. Shake the capped bottle of AddaVax before opening.
3. Mix equal volumes of antigen and AddaVax by pipetting.
The volume of injection depends of the site of administration. For example, 100-200 μl can be injected s.c. in mice.
Note: To avoid anaphylaxis, do not use adjuvants for intravenous injection.
Recommended maximum volumes for injection of antigen/adjuvant mixtures per site of injection for laboratory animals. (Lindblad EB., 2000. Freund’s Adjuvants. In: Vaccine adjuvants: Preparation Methods and Research Protocols. Humana Press.Totowa, NJ).