Active S. cerevisiae KEX2 protease protein fragment
Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.
Active S. cerevisiae KEX2 protease protein fragment from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX2. 1 milligram of recombinant KEX2 contains activity equivalent to at least 40 units
> 95 % by SDS-PAGE.Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.
Preservative: None Constituents: 5mM Calcium chloride, 10mM Sodium acetate, pH 6.0
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
Saccharomyces cerevisiae is a species of yeast. It is perhaps the most useful yeast, having been instrumental to winemaking, baking, and brewing since ancient times. It is believed that it was originally isolated from the skin of grapes (one can see the y
Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.
KEX 2; Kex2p; QDS1; SRB1; VMA45; S. cerevisiae KEX2; Saccharomyces cerevisiae KEX2