S. cerevisiae Active KEX2 protease protein (DAG-P2974)

S. cerevisiae Active KEX2 protease protein (aa 114 - 667), recombinant protein from High-5 cells

Product Overview
Active S. cerevisiae KEX2 protease protein fragment
Nature
Recombinant
Tag/Conjugate
Unconjugated
Molecular Weight
60 kDa
Cellular Localization
Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.
Bio-activity
Active S. cerevisiae KEX2 protease protein fragment from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX2. 1 milligram of recombinant KEX2 contains activity equivalent to at least 40 units
Procedure
None
Purity
> 95 % by SDS-PAGE.Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.
Format
Lyophilised
Buffer
Preservative: None Constituents: 5mM Calcium chloride, 10mM Sodium acetate, pH 6.0
Preservative
None
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Reconstitution
The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
Introduction
Saccharomyces cerevisiae is a species of yeast. It is perhaps the most useful yeast, having been instrumental to winemaking, baking, and brewing since ancient times. It is believed that it was originally isolated from the skin of grapes (one can see the y
Antigen Description
Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.
Keywords
KEX 2; Kex2p; QDS1; SRB1; VMA45; S. cerevisiae KEX2; Saccharomyces cerevisiae KEX2

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