HIV type 1 Active Reverse Transcriptase (full length), recombinant protein from E. coli
Active HIV1 Reverse Transcriptase full length protein
Specific activity determined as 10,000 to 20,000 units/mg. Definition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37°C.
> 90 % by SDS-PAGE.
Preservative: None Constituents: 50% Glycerol, 0.1% Triton-X-100, 50mM Sodium chloride, 5mM Magnesium chloride, 40mM Tris HCl, 10mM Beta mercaptoethanol, pH 8.3
Aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.
The human immunodeficiency virus (HIV) is a lentivirus (slowly replicating retrovirus) that causes the acquired immunodeficiency syndrome (AIDS), a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic
HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
HIV 1 Reverse transcriptase; HIV 1 RT; HIV RT; HIV1 RT; Human Immunodeficiency virus 1; Immuno Deficiency Virus Reverse Transcriptase
p51 RT; p66 RT; Reverse transcriptase p51 subunit; Reverse transcriptase p66 subunit; HIV1 RT