E. coli Active slyD (DAG-P2452)

E. coli Active slyD (aa 1 - 196), recombinant protein from E. coli Datasheet

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Product Overview
Active E. coli slyD full length protein
Nature
Recombinant
Tag/Conjugate
Unconjugated
Molecular Weight
21 kDa
Cellular Localization
Cytoplasmic
Bio-activity
Specific activity is >220 nmoles/min/mg.
Purity
>95% by SDS-PAGE .
Format
Liquid
Buffer
pH: 7.50Constituent: 0.32% Tris HCl
Storage
Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles. pH: 7.50Constituent: 0.32% Tris HCl This product is an active protein and may elicit a biological response in vivo, handle with caution.
Introduction
Escherichia coli; commonly abbreviated E. coli) is a gram-negative, facultatively anaerobic, rod-shaped bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harml
Antigen Description
slyD belongs to the FKBP-type PPIase family.Required for lysis of phiX174 infected cells. It binds nickel and zinc with high affinity and 1:1 stoichiometry, copper and cobalt with lower affinity. No binding is detectable for ferrous, ferric, magnesium and calcium ions. The activity of slyD is considerably smaller than the one found in other PPIases with the same substrate. The substrate specificity carried out with 'suc-Ala-Xaa-Pro-Phe-4NA', where Xaa is the AA tested, was found to be Phe > Ala > Leu.
Keywords
FKBP type peptidyl prolyl cis trans isomerase slyD; Histidine rich protein; PPIase; Rotamase; WHP; E. coli slyD; Escherichia coli slyD

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