E. coli Active BirA (DAG-P2273)

E. coli Active BirA (aa 2 - 321), recombinant protein from E. coli Datasheet

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Product Overview
Active E. coli BirA full length protein
Nature
Recombinant
Tag/Conjugate
Unconjugated
Molecular Weight
37 kDa including tags
Bio-activity
Active E. coli BirA full length protein (10 μg/ml) completely biotinylated 10 μM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 μM d-Biotin.
Purity
>= 65 % by SDS-PAGE.
Format
Liquid
Buffer
Preservative: 1.02% ImidazoleConstituents: 0.05% DTT, 0.79% Tris HCl, 5% Glycerol, 0.29% Sodium chloride
Storage
Upon delivery aliquot. Store at -80°C. Avoid freeze/thaw cycle.
Introduction
Escherichia coli; commonly abbreviated E. coli) is a gram-negative, facultatively anaerobic, rod-shaped bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harml
Antigen Description
BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.
Keywords
Bifunctional protein birA; Bio R; BioR; Biotin [acetyl CoA carboxylase] synthetase; Biotin operon repressor; Biotin protein ligase; Bir A; BirA bifunctional protein; Dhb B; dhbB; E. coli BirA; Escherichia coli BirA

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