Apple stem grooving virus (ASGV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect ASGV in infected herbaceous host plant tissues. Detection of ASGV in infected woody plant tissues are not consistent, and are depending on the viral titers in the sample tissues.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:30 -1:90.

General Description

Apple stem grooving virus (ASGV) is wide spread in its occurrence, and has been described as a latent virus of apple because it produces no symptoms on most commonly used commercial rootstocks and varieties. It was identified as a causal agent of apple top working disease when infected apple trees were grafted onto rootstocks of Malus sieboldii and Malus sieboldii var. arborescens.
Apple stem grooving virus is a flexuous filamentous virus estimated to be 600-700 nm x 12 nm in size, encapsidating a positive-sense single stranded RNA 6496 nucleotides in length excluding the poly (A) tail at the 3′end of the genome. ASGV is a member of the plant virus family flexiviridae, and the type member of the genus capillovirus. Members of genus capillovirus typically contain 2 open reading frames (ORFs): with ORF1 coding for the replicas protein at its 5′ end and CP at the 3′end of the ORF. ORF2 codes for a putative movement protein (MP), and is nested with ORF1.

Standard Curve