General Notes
This product (Catalog No. CABT-BL270) is a rabbit-derived polyclonal antibody raised against human AMFR, targeting the amino acid region 301–643 of the protein. This antibody exhibits cross-reactivity with Human AMFR from mouse and rat. It has been affinity purified by Protein A. AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).
CABT-BL270 is suitable for use in Western blot (WB) (1:1,000–1:8,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:200–1:800), IF/ICC (1:50–1:500), flow cytometry (FC) (0.40 μg per 10^6 cells in a 100 μl suspension) applications.
CABT-BL270 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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