Alfalfa latent virus (ALV) ELISA Kit

Name: Alfalfa latent virus (ALV) ELISA Kit
SKU: DEIAPV51
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

host plants

Intended Use

The test can be used to detect PeSV in infected pea, bean and other infected host plant tissues.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.

General Description

Alfalfa latent virus (ALV), a member of the carlavirus group, was isolated From alfalfa (Medicago sarivo) clones from rbe University of Nebraska experimental plots and from the Farmers' fields. The pea aphid, Acvrihosiphon pisum, transmitted this virus to M. sativa, Pisum sativum, and Viria faba. but not to Trifolium pratense. It was also sap transmissible to V. faba, V. villoso, and P. sarivum, but was not seed-borne in P. sariuum. In V. Jaba sap ALV was infectious at 65 but not at 70 C in 10 min. or alter 4 days but not after 6 days at 25 C and after dilution to 10-3 but not 10-4 Light microscopy indicated absence of any inclusion bodies in the epidermal strips of V. faba and P. surivum. With tobacco mosaic virus and a diffraction grating replica as standards, modal lengths of 653 and 635 nm, respectively, were determined for ALV. Serological and host range studies indicate that ALV is unrelated to pea streak virus, rcd clover vein mosaic virus, and cowpea mild mottle virus, other naturally occurring legume carlaviruses.
Symptoms by viral infection include necrotic lesions or streaks. Symptoms persist, or disappear soon after infection (in the glasshouse).

Standard Curve

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Avian leukosis virus subgroup - J as a contaminant in live commercially available poultry vaccines distributed in Nigeria

BIOLOGICALS

Authors: Shittu, Ismaila; Adedeji, Adeyinka J.; Luka, Pam D.; Asala, Olayinka O.; Sati, Nancy M.; Nwagbo, Ijeoma O.; Chinyere, Chinonyerem N.; Arowolo, Oluwadamilola O.; Adole, Jolly A.; Emennaa, Paulinus; Abdu, Paul A.; Joannis, Tony M.

Abstract

Globally, vaccines are used to prevent and control the menace of infectious diseases in livestock with some reported to be inadvertently contaminated with extraneous agents (EAs). With the aim of screening and characterizing for some selected EAs, 44 live viral poultry vaccines were randomly selected based on availability. The vaccines comprised 14 manufacturers in 10 different countries including Nigeria were screened by Polymerase Chain Reaction. In 9% (4/44) of the vaccines, contamination with only avian leukosis virus (ALV) subgroup J (ALV-J) was recorded. Other exogenous ALV subgroups, chicken infectious anemia and infectious laryngotracheitis viruses were absent. The EAs was found in infectious bursal disease (n = 1), Fowlpox (n = 2) and Mareks disease (n = 1) vaccines. Phytogenetic analysis of the ALV-J env gene showed clustering with contemporary group I and II. The result underscores the importance of screening vaccines to avoid the introduction and spread of EAs that could pose a threat to poultry production.

Diversity and evolution analysis of glycoprotein GP85 from avian leukosis virus subgroup J isolates from chickens of different genetic backgrounds during 1989-2016: Coexistence of five extremely different clusters

ARCHIVES OF VIROLOGY

Authors: Wang, Peikun; Lin, Lulu; Li, Haijuan; Yang, Yongli; Huang, Teng; Wei, Ping

Abstract

ALV-J has caused the most serious losses to the poultry industry in China. The gp85-coding sequence of ALV-J is known to be prone to mutation, but any association between the gp85 gene and breed of chicken remains unclear. A comprehensive and systematic study of the evolutionary process of ALV-J in China is needed. In this study, we compared and analyzed gp85 gene sequences from 198 ALV-J isolates, originating from China, USA, UK and France during 1989-2016. These were sorted into five clusters. Cluster 1, 2, 3, 4 and 5 included isolates from chicken types of different genetic backgrounds, e.g. white-feather broiler, Guangxi indigenous chicken breeds, Yellow chickens and layer chickens respectively. A correlation comparison of amino acid sequence similarities in the gp85 protein among the five clusters showed significant differences (P < 0.01) with the exception being when the third and fifth cluster were compared (P > 0.05). Results of entropy analysis of the gp85 sequences revealed that cluster 3 had the largest variation and cluster 1 had the least variation. The N-glycosylation sites in the majority of isolates numbered 14, 16, 17, 16 and 16, respectively, with regards to clusters 1-5. In addition, 5 isolates from cluster 3 had one more glycosylation site than the other isolates from cluster 3. Our study provides evidence that there were five extremely different ALV-J clusters during 1989-2016 and that the gp85 genes isolated from indigenous chicken breed isolates had the largest variation.