Mouse Alanine Aminotransferase (ALT/GPT) ELISA Kit

Name: Mouse Alanine Aminotransferase (ALT/GPT) ELISA Kit
SKU: DEIABL-M5
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma, Cell culture supernatant, Cell or tissue lysate, Other biological samples

Species Reactivity

Mouse

Detection Method

sELISA

Intended Use

For in vitro quantitative determination of Gpt/Alt concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

1. ELISA Microplate (Dismountable) - 8 wells x 12 strips
2. Lyophilized Standard - 2 vials
3. Biotin-labeled Antibody (Concentrated, 100X) - 1 vial, 120 μL
4. HRP-Streptavidin Conjugate (SABC, 100X) - 1 vial, 120 μL
5. TMB Substrate - 1 vial, 10 mL
6. Sample Dilution Buffer - 1 vial, 20 mL
7. Antibody Dilution Buffer - 1 vial, 10 mL
8. SABC Dilution Buffer - 1 vial, 10 mL
9. Stop Solution - 1 vial, 10 mL
10. Wash Buffer (25X) - 1 vial, 30 mL
11. Plate Sealer - 5 pieces
12. Product Description - 1 copy

Storage Condition for Opened Kit:
Put unused strips into a sealed foil bag with desiccant. Store for 1 month at 2-8 °C or 12 months at -20 °C.
Put remaining standards into a desiccant bag. Store for 1 month at 2-8 °C or 12 months at -20 °C.
All other reagents: 2-8 °C (avoid direct light for SABC).

Storage

2-8 °C (for sealed box), please do not freeze. See kit label for expiry date.

Performance Characteristics

Intra-assay Precision: Low, medium, high concentration samples tested 20 times on same plate.
Inter-assay Precision: Low, medium, high concentration samples tested 20 times on three different plates.

Recovery tested by adding known amount of Gpt/Alt to samples.

Linearity tested by diluting samples at 1:2, 1:4, and 1:8.

Stability tested at 37 °C for 1 month and 2-8 °C for 6 and 12 months.

Precision

Intra-assay Precision (n=20):
Sample 1: Mean 0.58 mIU/mL, SD 0.02, CV 4.23%
Sample 2: Mean 2.35 mIU/mL, SD 0.14, CV 5.91%
Sample 3: Mean 9.46 mIU/mL, SD 0.50, CV 5.26%

Inter-assay Precision (n=20):
Sample 1: Mean 0.61 mIU/mL, SD 0.03, CV 4.68%
Sample 2: Mean 2.46 mIU/mL, SD 0.13, CV 5.12%
Sample 3: Mean 9.54 mIU/mL, SD 0.45, CV 4.69%

Detection Range

0.313-20 mIU/mL

Detection Limit

0.188 mIU/mL

Sensitivity

0.188 mIU/mL

General Description

This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti Gpt/Alt antibody was pre-coated onto the 96-well plate. The biotin conjugated anti Gpt/Alt antibody was used as the detection antibody. The kit is designed for quantitative determination of Gpt/Alt in mouse biological samples.

Standard Curve

This product has been tested and meets performance specifications. (Laboratory humidity 20%-60%, temperature 18-25 C. TMB equilibrated to 37 °C before color development, incubated at 37 °C for 15 min.)

The following assay data are for reference only:

STD (mIU/mL)	OD-1	OD-2	Average	Corrected
0	0.050	0.052	0.051	0.000
0.313	0.120	0.124	0.122	0.071
0.625	0.236	0.242	0.239	0.188
1.250	0.390	0.402	0.396	0.345
2.500	0.623	0.641		0.581
5.000	0.859	0.883	0.871	0.820
10.000	1.447	1.489	1.468	1.417
20.000	2.173	2.237	2.205	2.154

Reconstitution And Storage

For Standards:
1. Centrifuge standard tube for 1 min at 10000 x g.
2. Add 1 mL sample dilution buffer into standard tube. Tighten cap and let stand for 2 min at room temperature. Invert several times to mix gently.
3. Centrifuge for 1 min at 1000 x g to bring liquid to bottom.
Note: Store dissolved zero tube standard at 2-8 °C and use within 12 h. Diluted working standards should be used within 2 h.

For Wash Buffer:
Dilute 30 mL concentrated wash buffer to 750 mL with deionized or distilled water (recommended resistivity: 18 MΩ). Mix well.
Crystals can be dissolved by heating at 40 °C water bath (below 50 C).
Prepared wash buffer: best used same day; can store at 2-8 °C within 48 h.

Citations