ADAM20 peptide

Name: ADAM20 peptide
SKU: DAG-P0128
Rating: 5 (1 reviews)

Synthetic ADAM20 peptide for ELISA, WB

COA

SDS

Datasheet

Summary

Specificity

Testis specific.

Nature

Synthetic

Tag/Conjugate

Unconjugated

SequenceSimilarities

Contains 1 disintegrin domain.Contains 1 EGF-like domain.Contains 1 peptidase M12B domain.

Alternative Names

A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg

Purity

> 95 % by SDS-PAGE.

Format

Liquid

Buffer

Preservative: None Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75

Preservative

None

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Preservative: None Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75

Entrez Gene ID

8748

Antigen Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The expression of this gene is testis-specific. [provided by RefSeq, Jul 2008]

Function

metalloendopeptidase activity; metallopeptidase activity; zinc ion binding;

Synonyms

ADAM20; ADAM metallopeptidase domain 20; disintegrin and metalloproteinase domain-containing protein 20; ADAM 20; a disintegrin and metalloproteinase domain 20;

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Sperm-egg fusion disorder in a Chinese male patient was associated with a rare ADAM20 variant

ONCOTARGET

Authors: Sha, Yan-Wei; Xu, Xiaohui; Ji, Zhi-Yong; Mei, Li-Bin; Qiu, Ping-Ping; Ji, Hong; Li, Ping; Li, Lin; Liu, Wei-Wu

Abstract

We report here a 28-year-old male with infertility. No abnormality was found in his semen examination. The couple achieved a successful pregnancy under the help of intracytoplasmic sperm injection during which we found that sperm could enter the zona pellucida, but could not fuse with the egg within the short insemination period. We then performed whole-exome sequencing technology on this patient and found a rare variant (c. 641A> C: p. D214A) in ADAM20, which encoded a disintegrin and metalloprotease 20 protein. To further verify the pathogenicity of this variant, we analyzed ADAM20 protein expression in spermatozoa by immunostaining analysis, which showed a mis-localization of ADAM20 in the patient's spermatozoa. Therefore, we concluded that mutation in ADAM20 may be associated with sperm-egg fusion disorder in this patient.