ACTH (Adrenocorticotropic Hormone) ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The ACTH ELISA is intended for the quantitative determination of ACTH (Adreno-corticotropic Hormone) in human plasma. This assay is intended for research use only.

Contents of Kit

Storage

Store all kit components at 2-8°C except Wash Concentrate and Stop Solution.

Performance Characteristics

Accuracy
Three hundred (300) samples, with ACTH values ranging from 1.0 to 640 pg/mL were assayed by the previous ACTH kit and the updated ACTH kit ELISA. Linear regression analysis gives the following statistics:
ACTH ELISA = 1.02 - 1.58 pg/mL r = 0.995 N = 300

Precision

The precision (intra-assay variation) of the ACTH ELISA Test was calculated from 25 replicate determinations on each of the two samples.
Intra-Assay Variation

The total precision (inter-assay variation) of the ACTH ELISA Test was calculated from data on two samples obtained in 21 different assays, by three technicians on three different lots of reagents, over a four-week period.
Inter-Assay Variation

Sensitivity

The sensitivity, or minimum detection limit, of this assay is defined as the smallest single value, which can be distinguished from zero at the 95% confidence limit. The ACTH ELISA has a calculated sensitivity of 0.22 pg/mL.