Anti-ACLSV polyclonal antibody

In Sicily, the peach industry, based on local germplasm, seedling propagated has been documented since the XIII century. In 1998, in order to study the horticultural traits of this germplasm, an ex situ collection of twenty-two genotypes found all around the island has been set up. To check the sanitary status and fit the European Union (EU) rules for plant material certification, a preliminary analysis for PPV, PDV, PNRSV, ACLSV, ApMV and PLMVd has been carried out. From a horticultural point of view, the collected genotypes ripe from July ('Red Heaven' time) up to the end of October. Most of them are white-fleshed, clingstone, and with high soluble solids content. Fifty percent of the accessions were pathogen free.

The genome of the Balaton1 severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV-Bal1) has been cloned and sequenced. The genomic RNA is 7 549 nucleotide long, excluding the poly A tail. The genomic organization, with three overlapping open reading frames (ORF), is similar to that of the other sequenced ACLSV isolates. Sequence comparisons indicate a high variability between ACLSV isolates, with overall nucleotide sequence homology levels between 76 and 82%. The coat protein, encoded internally inside a larger ORF, is the most conserved protein (identity levels between 87 and 93%) while the central ORF, encoding the putative movement protein, is the most divergent (77 to 85% identity).