cell culture supernatants, purified virus preparations
Enzyme Immunoassay for the Quantitative Determination of AAV Serotype 5 Particles in Cell Culture Supernatants and Purified Virus Preparations.
Contents of Kit
1. Microtiter Plate
2. Kit Control (empty AAV 5 capsids)
3. Sample Buffer, 20X
4. Wash Buffer 20X
5. Anti-AAV 5 Biotin Conjugate 20X
6. Streptavidin Peroxidase Conjugate 20X
7. Substrate;tetramethylbenzidine (TMB) in methylpyrrolidone
8. Stop Solution
For more detailed information, please download the following document on our website.
Adeno-associated virus (AAV) is a non pathogenic ssDNA virus that is a topic of intense study in gene therapy. The virus transduces a wide variety of dividing and non-dividing cells showing long-term gene expression with no cellular immune response. AAV has been used in several clinical trials (e.g. FIX, CFTR, Parkinson' s disease, Canavan disease) showing no serious vector-related adverse effects. Methods for the characterisation of AAV preparations currently include titration ELISA, realtime PCR, DNA dot blot, determination of transducing units, infectious center assay, SDS-PAGE or electron microscopy.
Immunotitration by AAV5 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV5 wt virions, AAV5 recombinant virions or assembled and intact empty AAV5 capsids.