Shipped at room temperature and it should be stored at room temp. DO NOT FREEZE. Stable for 6 months.
Adsorption of most proteins can be achieved by mixing protein solution with Alhydrogel (1:1 or 1:10 v/v ratio of gel:protein can be tested) with gentle mixing at room temperature for 2-24 hrs. Protein left in the supernatant can be measured by protein assay or specific ELISA. Alhydrogel adsorbed proteins can be injected into animals at a desired dose. If diluting the stock adsorbed-protein gel, volume can be adjusted with non-adsorbed gel to keep the same amount of gel. In veterinary vaccines there is no defined maximum limit for the allowed content of aluminium adjuvants.
Alhydrogel 2% has worldwide acceptance and it is used in many vaccines. Alhydrogel is the standard preparations for immunological research on aluminum hydroxide gels. The use of aluminum adjuvants is accompanied by stimulation of IL-4 and stimulation of the T-helper-2 subsets in mice, with enhanced IgG1 and IgE production. Aluminum hydroxide gel is obtained by precipitation of aluminum hydroxide Al(OH)3 under alkaline conditions. It is currently used as an adjuvant in subunit, DNA and conjugate vaccines. Aluminium content: 9.0 – 11.0 mg/ml.pH: 6-7 Binding capacity: 10-20 mg human serum albumin per ml gel at pH 7.0. Note: Protein binding will vary with protein concentration, buffer and incubation conditions.
Al(OH)3, Aluminium hydroxide gel. White gelatinous precipitate. Alhydrogel 2% is supplied as ready to use, gel suspension, sterile solution.