2’3’-cGAMP

Content
1 mg (2 x 500 μg) lyophilized 2’3’-cGAMP Vaccine grade adjuvant.
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Storage and Stability
2’3’-cGAMP is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year when properly stored.
Upon resuspension, prepare aliquots of 2’3’-cGAMP and store at -20°C. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.

Description
2’3’-cGAMP (cyclic [G(2’,5’)pA(3’,5’)p]) is a cyclic dinucleotide produced in mammalian cells by cGAS (cGAMP synthase) in response of double-stranded DNA in the cytoplasm. Cyclic dinucleotides (CDNs) are a relatively new class of adjuvants that have been shown to increase vaccine potency. CDNs activate innate immunity by directly binding the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes), activating a signaling pathway that induces the expression of interferon-β (IFN-β) and also nuclear factor-κB (NF-κB) dependent inflammatory cytokines.
2’3’-cGAMP is also referred to as “noncanonical” cGAMP due to the presence of the atypical 2’-5’ phosphodiester linkage between the guanosine and the adenosine. Structural and functional studies revealed that noncanonical 2’3’-cGAMP is distinct from the canonical 3’3’-cGAMP produced by bacteria. Certain variants of STING are able to distinguish between noncanonical and canonical cGAMP.
Similar to the canonical 3’3’-cGAMP, 2’3’-cGAMP serves as a second messenger to activate innate immune responses by binding to STING and subsequently inducing the TBK1-IRF3 (TANK-binding kinase 1-IFN regulatory transcription factor3)-dependent production of IFN-β. Recently, it has been reported that 2’3’-cGAMP is an effective adjuvant that boosts the production of antigen-specific antibodies and T cell responses in mice.

Preparation of Stock Solution (1 mg/ml)
Add 500 μl sterile endotoxin-free physiological water (provided) to 500 μg 2’3’-cGAMP.
Mix the solution by pipetting up and down.

Vaccine Grade