17b-19-Nortestosterone(3) [HRP]

Nandrolone and other 19-norsteroid potent anabolic steroids have been prohibited in sports for 30 years. The detection of the main urinary metabolite - 19-norandrosterone - in amounts greater than 2 ng/ml constitutes an adverse analytical finding. The presence in nutritional sport supplements of steroids not listed on the label has undoubtedly resulted in positive tests, but inadvertent consumption of meat containing residues of hormonal treatment should not realistically cause apprehension. Although highly improbable, athletes should prudently avoid meals composed of pig offal in the hours preceding the test since the consumption of edible parts of a non-castrated pig, containing 19-nortestosterone, has been shown to results in the excretion of 19-norandrosterone in the following hours. Norsteroid metabolites are formed during pregnancy and excreted as minor metabolites of norethisterone, and minute amounts have been identified in some male and female samples when using more sensitive techniques of detection. Whereas exercise does not seem to be a significant factor in 19-norandrosterone excretion, some rare urine samples were found to be a suitable medium for in situ 19-demethylation of urinary metabolites.

Progestins (PGs) are a group of steroid hormones known to have endocrine-disrupting effects. These compounds can enter the aquatic environment via the discharge of treated or untreated wastewater and the disposal of sludge from sewage treatment plants (STPs); thus, their removal in STPs are of great importance. The present study simultaneously investigated the occurrences and fates of 62 PGs in a municipal STP in Beijing, China. Progesterone (P) and its metabolites were found to be the predominant compounds, with total dissolved concentrations of 1866 ng/L in the influent. About 11 P metabolites were newly detected, accounting for 25-55% and 75-91% of the total concentrations in wastewater and sludge, respectively. For the other three groups of PGs derived from different parent compounds, P derivatives were first detected in the STP with the highest concentration in the wastewater and sludge, followed by 19-nortestosterone (NT) derivatives and 17 alpha-hydroxyprogesterone (17 alpha-OHP) derivatives. The removal efficiencies in the dissolved phase of wastewater were relatively high for P and its metabolites (95-99%) and P derivatives (91-99%). And the relative persistence of NT (68-99%) and 17 alpha-OHP derivatives (79-99%) was observed during the wastewater treatment processes. Mass balance analysis showed that the lost mass proportions were as high as 41-99%, the mass fractions in sludge were in the range of 0-55%, and 0.24-25% of the initial mass loadings was present in the effluent. These results indicated that biodegradation was the major removal mechanism of PGs in the STP. (C) 2019 Elsevier Ltd. All rights reserved.