17a-Estradiol(3) [HRP] (DAG1181)

17a-Estradiol 3 conjugate with HRP for use in immunoassays

Target
Estriol
Nature
Synthetic
Tag/Conjugate
HRP
Alternative Names
17alpha-Estradiol; alpha-Estradiol; Epiestradiol; Alfatradiol
Format
Concentrate
Concentration
Please refer to the vial lable for the specific concentration.
Size
500 μL
Buffer
Supplied as a concentrate. Dilute as required and use working strength conjugate immediately after dilution.
Storage
Store at 2-8 °C for up to 3 months or -20 °C for long term storage
Ship
Wet ice
Warnings
This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations.
Antigen Description
17alpha-estradiol is an estradiol that is estra-1,3,5(10)-triene substituted by hydroxy groups at positions 3 and 17 (the 17alpha stereoisomer). It is a 17alpha-hydroxy steroid, a 3-hydroxy steroid and an estradiol.

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References


Development of matrix effect-free MISPE-UHPLC-MS/MS method for determination of lovastatin in Pu-erh tea, oyster mushroom, and red yeast rice

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Authors: Svoboda, Pavel; Sander, Daniel; Plachka, Katerina; Novakova, Lucie

Matrix effect-free UHPLC-MS/MS method was developed and validated for the determination of cholesterol-lowering lovastatin in food samples represented by Pu-erh tea, oyster mushroom, and red yeast rice. The resulting method was fully validated in terms of intra-day and inter-day precision, accuracy, linearity, range, LOD, LOQ and matrix effects. The matrix effect phenomenon evaluated by comparison of slopes of calibration curves was completely eliminated by solid-phase extraction based on the technique of molecularly imprinted polymers (MIPs). Comparison of elution profiles obtained on the MIP and corresponding control non-imprinted polymer (NIP) showed selectivity of the extraction procedure. In addition, selectivity of the MIP material and the molecularly imprinted solid-phase extraction (MISPE) was also proved by experiments evaluating retention of analytes physico-chemically similar to the target molecule. Extraction recoveries of these analytes represented by estrogen derivatives (estrone, estriol, 17 alpha-ethinylestradiol, and (beta-estradiol) were very low or even null. Synthesis and preparation of the resulting MIP sorbent was characterized by excellent repeatability expressed as RSD 7.7% (n = 9) of extraction recoveries. The determined capacity of the MIP material reaching 375 ng/mg is sufficient for analysis of the evaluated statin in its natural sources. Suitability of the resulting MISPE-UHPLC-MS/MS procedure for real sample analysis was verified by the determination of lovastatin in one dietary supplement based on the red yeast rice with a given amount of the target analyte. Finally, three mushroom and fifteen tea samples obtained in Czech food stores and tearooms were subjected to analysis. Low or null amount of lovastatin was found in these samples. (C) 2017 Elsevier B.V. All rights reserved.

An alternative perspective of hollow fiber-mediated extraction: Bundled hollow fiber array-liquid-phase microextraction with sonication-assisted desorption and liquid chromatography-tandem mass spectrometry for determination of estrogens in aqueous matrices

JOURNAL OF CHROMATOGRAPHY A

Authors: Goh, Shalene Xue Lin; Lee, Hian Kee

A bundled hollow fiber array (BHF)-liquid-phase microextraction (LPME) approach has been developed for the ultra-high performance liquid chromatography tandem mass spectrometric determination of estrone, 17 beta-estradiol, estriol, and 17 alpha-ethinylestradiol. The BHF was dipped in n-octanol to impregnate only the wall pores of the hollow fibers without deliberate loading of extractant solvent in the lumens, before placing it in the sample for extraction. Parameters influencing extraction efficiency, such as number of bundled hollow fibers, type of extraction and desorption solvent, agitation mode, extraction temperature and duration, and the salting out effect were examined. Under the most favourable experimental conditions, the enrichment factors were between 77 and 137-fold for the target compounds. The developed method offered high sensitivity and reproducibility. Low limits of detection and limits of quantification were achieved, i.e., between 0.251 and 0.440 ng/L, and 0.995 and 1.82 ng/L, respectively. Good intra and inter-day precision were obtained with relative standard deviations (RSDs) of less than 9%. BHF-LPME provided a new perspective on using membrane solvent-supported hollow fiber-mediated LPME. Raman spectroscopy was also introduced in this study to reaffirm that the polypropylene BHF did not play any role in the extraction; only the solvent was responsible. (C) 2017 Elsevier B.V. All rights reserved.

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