β-Defensin 2 ELISA Kit (DEIA040J)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
stool
Species Reactivity
Human
Intended Use
This assay is intended for the quantitative determination of β-defensin 2 in stool.
Contents of Kit
1. Holder with precoated strips: 12 x 8 wells
2. ELISA Wash Buffer concentrate 10X: 2 x 100 mL
3. Conjugate concentrate, (Streptavidin, HRP-conjugated): 200 μL
4. Standards, lyophilized: 2 x 5 vials
5. Standard dilution buffer: 20 mL
6. Control 1, lyophilized: 2 x 1 vial
7. Control 2, lyophilized: 2 x 1 vial
8. Extraction buffer concentrate 2.5X: 2 x 100 mL
9. TMB substrate (tetramethylbenzidine), ready to use: 15 mL
10. ELISA stop solution, ready to use: 15 mL
Storage
Store the kit at 4°C upon receipt. For more detailed information, please download the following document on our website.
Precision
Intra-assay Precision: 3.0%-4.1%
Inter-assay Precision: 8.1%-9.1%
Sensitivity
0.02 ng/mL; 2 ng/mL

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References


Tissue-engineered human psoriatic skin supplemented with cytokines as an in vitro model to study plaque psoriasis

REGENERATIVE MEDICINE

Authors: Pouliot-Berube, Claudia; Zaniolo, Karine; Guerin, Sylvain L.; Pouliot, Roxane

Aim: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used. Materials & methods: Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-alpha, IL-1 alpha, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray. Results: Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU). The expression of those genes was dramatically increased compared with basal levels of controls (p < 0.005 to < 0.05). Conclusion: Psoriatic substitutes supplemented with a cocktail of TNF-alpha, IL-1 alpha, IL-6 and IL-17A showed similar transcriptome alterations to those found in psoriasis.

Transcriptional landscape of psoriasis identifies the involvement of IL36 and IL36RN

BMC GENOMICS

Authors: Keermann, Maris; Koeks, Sulev; Reimann, Ene; Prans, Ele; Abram, Kristi; Kingo, Kuelli

Background: In present study we performed whole transcriptome analysis in plaque psoriasis patients and compared lesional skin with non-lesional skin and with the skin from healthy controls. We sequenced total RNA from 12 lesional (LP), 12 non-lesional (NLP) and from 12 normal (C) skin biopsies. Results: Compared with previous gene expression profiling studies we had three groups under analysis - LP, NLP and C. Using NLP samples allows to see the transcriptome of visually normal skin from psoriasis patient. In LP skin S100A12, S100A7A, LCE3E, DEFB4A, IL19 were found up regulated. In addition to already these well-described genes, we also found several other genes related to psoriasis. Namely, KLK9, OAS2, OAS3, PLA2G, IL36G, IL36RN were found to be significantly and consistently related to the psoriatic lesions and this finding is supported also by previous studies. The genes up-regulated in the LP samples were related to the innate immunity, IL17 and IL10 networks. In NLP samples innate immunity and IL17 network were activated, but activation of IL10 network was not evident. The transcriptional changes characteristic in the NLP samples can be considered as a molecular signature of "dormant psoriasis". Conclusions: Taken together, our study described the transcriptome profile characteristic for LP and NLP psoriatic skin. RNA profile of the NLP skin is in between the lesional and healthy skin, with its own specific pattern. We found that both LP and NLP have up-regulated IL17 network, whereas LP skin has up regulated IL10 related cytokines (IL19, IL20, IL24). Moreover, IL36G and IL36RN were identified as strong regulators of skin pathology in both LP and NLP skin samples, with stronger influence in LP samples.

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