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Protein L [HRP]   (DAG4610)  


Protein L [HRP], recombinant protein           Datasheet

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Product Overview
Recombinant protein L, HRP-conjugated
Nature
Recombinant
Tag/Conjugate
HRP
Purity
> 95% (Evalutated by HPLC)
Format
Liquid PBS density is 20mm. PH 7.4
Concentration
1 ml (0.5 mg/ml protein L)
Storage
2-8°C short term, -20°C long term
Introduction
Protein L was first isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind immunoglobulins through L chain interaction, from which the name was suggested. It consists of 719 amino acid residues. The molecular weight of Protein L purified from the cell walls of Peptostreptoccus magnus was first estimated as 95kD by SDS-PAGE in the presence of reducing agent 2-mercaptoethanol, while the molecular weight was determined to 76kD by gel chromotography in the presence of 6 M guanidine HCl. Protein L does not contain any interchain disulfide loops, nor does it consist of disulfide-linked subunits. It was an acidic molecular with a PI of 4.0. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobilins (antibodies), Protein L binds antibodies through light chain interactions. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.
Keywords
Protein L

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Tel: 44-207-097-1828
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