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Ubiquitin/Proteasome Pathway Antibodies

Ubiquitin/Proteasome Pathway Antibodies

Creative Diagnostics now offers antibodies against full length or partial proteins, from the ubiquitin proteasome (UPS) pathway; these include E2 conjugating enzymes, E3 ligases, DUBs, ubiquitin binding proteins substrates and conjugate antibodies and also a number of kinase antibodies. Most of these antibodies can be used in WB and IP and can be supplied as high quality research tools to the life science research community.

Popular Ubiquitin / Proteasome Pathway Antibodies

Cat_No Description Source Application
CABT-BL6367 mono and polyubiquitylated conjugates Mab Mouse WB
CABT-BL6368 mono and polyubiquitylated conjugates Mab Mouse WB
CABT-BL6369 mono and polyubiquitylated conjugates Mab Mouse WB
CABT-BL6370 Polyubiquitylated conjugates Mab Mouse WB
DCABY-4229 Ubiquitin monoclonal antibody Mouse WB, ELISA(Cap)
DCABY-4303 Ubiquitin monoclonal antibody [Biotin] Mouse WB, ELISA(Det)
CABT-B1642 Ubiquitin (phospho S65) polyclonal antibody Rabbit WB, ICC
DPABH-22055 Ubiquitin B polyclonal antibody Rabbit IHC, ICC/IF
DPAB2686 Ubiquitin+1 polyclonal antibody Rabbit WB, IP
CABT-BL6311 Human ABIN 1 polyclonal antibody Sheep WB, IP
CABT-BL6312 Mouse ABIN 1 polyclonal antibody Sheep WB, IP
CABT-BL6313 Human FAF1 polyclonal antibody Sheep WB, IP
CABT-BL6314 Human NEMO polyclonal antibody heep IP
CABT-BL6315 Human Optineurin polyclonal antibody Sheep IP
CABT-BL6316 Human UBXD7 polyclonal antibody Sheep WB, IP
CABT-BL6317 Human CAND1 polyclonal antibody Sheep WB, IP
CABT-BL6318 Human Cullin 3 polyclonal antibody Sheep WB
CABT-BL6319 Human Cullin 5 (aa 577-689) polyclonal antibody Sheep WB, IP
ABT-BL6320 Human DCNL3 polyclonal antibody Sheep WB, IP
CABT-BL6321 Human DCNL4 polyclonal antibody Sheep WB, IP
CABT-BL6322 Human DCNL5 polyclonal antibody Sheep WB, IP
CABT-BL6323 Mouse DCNL5 polyclonal antibody Sheep WB, IP
CABT-BL6324 Human HOIL1 polyclonal antibody Sheep WB, IP
CABT-BL6325 Human HOIP polyclonal antibody Sheep WB, IP
CABT-BL6326 Human Parkin polyclonal antibody Sheep WB, IP
CABT-BL6327 Human RNF7 polyclonal antibody Sheep WB, IP
CABT-BL6328 Human SHARPIN polyclonal antibody Sheep WB, IP
CABT-BL6329 Mouse TRAF6 polyclonal antibody Sheep IP
CABT-BL6330 Human TRIAD1 polyclonal antibody Sheep WB, IP
CABT-BL6331 Human TRIM65 polyclonal antibody Sheep WB, IP
CABT-BL6332 Human CYLD polyclonal antibody Sheep WB, IP
CABT-BL6333 Human OTUB1 polyclonal antibody Sheep WB, IP
CABT-BL6334 Human OTULIN polyclonal antibody Sheep WB, IP
CABT-BL6335 Human USP11 polyconal antibody Sheep WB, IP
CABT-BL6336 Human USP15 polyclonal antibody Sheep WB, IP
CABT-BL6337 Human AMPK alpha 2 (aa 352–366) polyclonal antibody Sheep WB, IP
CABT-BL6338 IKK beta polyclonal antibody Sheep IP
CABT-BL6339 Human IKK epsilon (aa 701-716) polyclonal antibody Sheep WB, IP
CABT-BL6340 Human IKK epsilon (phospho S172) polyclonal antibody Sheep WB, IP
CABT-BL6341 Mouse IKK epsilon (aa 702-717) polyclonal antibody Sheep WB, IP
CABT-BL6342 Human MAPK15 polyclonal antibody Sheep WB
CABT-BL6343 PINK1 (aa 175-250) polyclonal antibody Sheep WB, IP
CABT-BL6344 Human PINK1 (phospho T257) polyclonal antibody Sheep WB
CABT-BL6345 Human PKB alpha polyclonal antibody Sheep IP
CABT-BL6346 Mouse PKB beta (aa 455-469) polyclonal antibody Sheep WB, IP
CABT-BL6347 Human PKB gamma (aa 116-127) polyclonal antibody Sheep IP
CABT-BL6348 Human SGK1 polyclonal antibody Sheep WB, IP
CABT-BL6349 Human SGK1 (aa 412-431) polyclonal antibody Sheep WB, IP
CABT-BL6350 Human SGK2 (aa 333-346) polyclonal antibody Sheep WB, IP
CABT-BL6351 Human SGK3 polyclonal antibody Sheep WB, IP
CABT-BL6352 Human TBK1 polyclonal antibdy Sheep IP
CABT-BL6353 Human TBK1 (Phospho S172) polyclonal antibody Sheep WB
CABT-BL6354 Human UBE2M polyclonal antibody Sheep WB
CABT-BL6355 Human UBE2T polyclonal antibody Sheep WB, IP
CABT-BL6356 Human FOXO3A (phospho S294) polyclonal antibody Sheep WB
CABT-BL6359 Human MYPT1 (aa 728 – 838) polyclonal antibody Sheep WB
CABT-BL6360 Mouse MYPT1 (phospho S445 ) polyclonal antibody Sheep WB
CABT-BL6365 Human MYPT2 (aa 350-450) polyclonal antibody Sheep WB
CABT-BL6366 Human MYPT3 polyclonal antibody Sheep WB

 

Ubiquitin is a small protein that is found in almost all cellular tissues in humans and other eukaryotic organisms, which helps to regulate the processes of other proteins in the body and can be found tagged to almost any protein. It is evolutionarily advantageous for all cells of eukaryotic organisms to use the process of ubiquitylation because it removes breaking, worn or sub-functional proteins from cells before the proteins cease functioning entirely. A system that pre-emptively removes proteins from use and tags them for protein recycling keeps cells healthy.

Ubiquitination, also known as ubiquitylation, is an enzymatic process that involves the bonding of an ubiquitin protein to a substrate protein. This has sometimes been referred to as the molecular “kiss of death” for a protein, as the substrate usually becomes inactivated and is tagged for degradation by the proteasome through the attachment of the ubiquitin molecule. Ubiquitination occurs when an ubiquitin molecule bonds to a substrate protein and is a type of post-translational modification.

This process involves three steps with specific groups of enzymes to perform them, which are:
     a. Activation with ubiquitin-activating enzymes (E1s)
     b. Conjugation with ubiquitin-conjugating enzymes (E2s)
     c. Ligation with ubiquitin ligases (E3s)

Ubiquitin/Proteasome Pathway Antibodies

The first step involves the activation of ubiquitin by the E1 enzyme, which occurs prior to its attachment to the amino acid cysteine, the active site. Energy in the form of ATP is required in order for the ubiquitin molecule to be transferred to the active site and produce an intermediate substance known as ubiquitin-adenylate. Following this, the ubiquitin-conjugating enzyme (E2) plays its role to bring the two molecules together, by transferring the ubiquitin from E1 to the active cysteine site. The E2 enzyme has a particular structure that allows it to bond to both the ubiquitin and E1 molecules and allow this step to occur. Finally, the ubiquitin protein ligase (E3) is required to recognise and bind the target substrate, subsequently labeling it with the small ubiquitin molecule. This usually occurs by way of an isopeptide bond connecting the last amino acid, glycine 76, of the ubiquitin molecule to a lysine on the substrate protein. This enzymatic process is then repeated to form a small chain with several ubiquitin molecules, marking the protein for degradation in the proteasome.

Ubiquitin is known to form covalent bonds with proteins, marking them in the body for degradation through the ubiquitin/ATP-pathway. This process plays a vital role in the healthy turnover of proteins in the body. When cells marked by ubiquitin bonding are not degenerated properly, the cellular homeostasis is disrupted and eventually this can result in the genesis of certain diseases. Including:

•  Neurofibrillary tangles (Alzheimer’s disease)

•  Lewy body (Parkinson’s disease)

•  Pick bodies (Pick’s disease)

•  Inclusions (Huntington’s disease and motor neuron disease)

•  Mallory bodies (alcoholic liver diseases)

•  Rosenthal fibers (astrocytes)

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