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14-3-3 theta/tau (Phospho-Ser232) ELISA Kit   (DEIA-XYA133)  


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Size
2 x 96T
Sample
cultured cells
Species Reactivity
Human, Mouse, Rat
Intended Use
The 14-3-3 θ/τ (Phospho-Ser232) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor 14-3-3 θ/τ protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated 14-3-3 θ/τ in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on 14-3-3 θ/τ phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-14-3-3 θ/τ (Phospho-Ser232) Antibody (Rabbit Polyclonal): 60 μL (100x), Red
7. 100x Anti-14-3-3 θ/τ Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x), Glass
11. Primary Antibody Diluent: 12 mL (1x), Clear
12. Ready-to-Use Substrate: 12 mL (1x), Brown
13. Stop Solution: 12 mL (1x), Clear
14. Crystal Violet Solution: 12 mL (1x), Glass
15. SDS Solution: 24 mL (1x), Clear
16. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months

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Tel: 1-631-624-4882
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United States
Tel: 1-631-624-4882
Fax: 1-631-938-8221
Europe
Tel: 44-207-097-1828
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