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In Situ Hybridization (ISH)

In Situ Hybridization (ISH) refers to localizing a specific DNA or RNA sequence in whole embryos or tissues/tissue sections/cells using a labeled probe. The probe is either a labeled complementary DNA (oligoprobe) or a complementary RNA (riboprobe).

Creative Diagnostics offers customized ISH service, including probe design, tissue procurement and expertly interpreted gene expression studies. Our scientists have successfully developed a fixative that preserves nucleic acid best and allows the most beautiful ISH results. Besides, our scientists have undergone extensive ISH training to bring you highly sensitive nucleic acid detection in the sample.

Featured Services:

Multiplex ISH

Multiplex ISH can be performed whereby multiple probes are used to simultaneously localize different sequences in the same tissue section. Creative Diagnostics has more options of probe design, labeling and purification.


Dual ISH application: normal cell (left) compared with cancer cell (right)


MicroRNA ISH

Creative Diagnostics has extensive experience with microRNA ISH using our or client’ probes. Besides, we offer in situ hybridization screening of your microRNAs using enhanced microRNA detection probes.

Combination ISH and IHC - This service allow you to quickly visualize your target protein and transcript in its native setting.


Combined IHC labeling (brown) with ISH for target RNA (blue)


With combined academic and industrial experiences, we are confident that we will achieve high quality work and guarantee you complete satisfaction. We are eager to learn of our customers' specific ISH requirement and to facilitate their research and product development.

Please feel free to contact us if you have any questions regarding our service.

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Tel: 1-631-624-4882
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