Creative Diagnostics has developed a successful immunization protocol improving the generation of polyclonal anti-idiotype antibodies (anti-IDs). In addition to the traditional mouse and rabbit options, we also offer goat, chicken, sheep, donkey, Guinea pig, Llama, Camel, horse, dogs, bovine, primate or host animals specified by the clients.
To enrich the antibodies that target the antigen-binding site of the original antibody or receptor, we usually cut the target antibody into Fab fragments to immunize animals (Antibody Fragmentation service). Once got the antiserum, we will perform multi-step affinity purification to ensure the isolated antibody’s specificity to the binding site of the antibody or receptor. Additionally, we can develop ELISA assays to support your PK/PD studies, and set up neutralization and blocking assay to identify functional ligand blocking antibodies/receptors.
Creative Diagnostics has produced functional polyclonal anti-IDs that have been extensively characterized. We could develop the antibodies that work in your end application, such as routine screening, ELISA assay, Immunohistochemistry, Western blot, Flow cytometry, etc. For more information, please check our Antibody Labeling and contact assay developmentservices.