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Monoclonal Anti-idiotypic Antibody Production

Monoclonal anti-idiotype antibodies are ideal for preclinical research (PK, Tox and immunogenicity study) and the generation of data to support the development of antibody drugs. Creative Diagnostics has developed high-quality monoclonal anti-idiotypic antibodies used in sandwich ELISA for quantification of drug antibodies in patient serum.

Monoclonal Anti-idiotypic Antibody Production

Our monoclonal anti-idiotype antibody solution is a highly customizable service based entirely on the customer needs. To complement the monoclonal anti-idiotype antibodies development, we offer following feature services:

  • Fast turnaround time
  • More than 95% success rate
  • Professional immunogenic design
  • Standard programs for affinity maturation
  • High-throughput cloning
  • High-throughput screening
  • Characterization assays

Monoclonal anti-idiotype antibody production packages

Item Basic Package Peptide Package Fab & F(ab)2 Package
Experimental scheme design (1-2 days)
Peptide synthesis up to 20 aa, HPLC purity > 90% (3-4 weeks)    
Peptide conjugation to carrier protein (KLH, BSA, OVA)    
Fab & F(ab)2 fragmentation and purification (3-4 weeks)    
Phase I: Immunization (4-6 weeks)
  • Immunization of 5 Balb/c mice
  • ELISA testing of serum
Phase II: Fusion (2-3 weeks)
  • Fusion of 1 mouse spleen with sp2/0
  • ELISA screening
Phase III: Sub-cloning (4-6 weeks)
  • 2-3 sub-cloning steps
  • ELISA screening
Isotyping of final clones
Antibody production (3-4 weeks)
  • Ascetic fluid
  • Protein A/G purification

Our Guarantees:

  • Timely feedback
  • Personalized customization service
  • Competitive price
  • High specificity and affinity antibodies development

Please contact us to understand more custom monoclonal anti-Idiotypic antibodies production services from Creative Diagnostics to meet your specific requirements.

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