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Human FGF-20 ELISA Kit   (DEIA-XYZ128)  


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Size
96T
Sample
Cell Culture Supernatants, Plasma, Serum
Species Reactivity
Human
Intended Use
The Human FGF-20 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF-20 in serum, plasma and cell culture supernatants.
Contents of Kit
1. Pre-Coated 96-well Strip Microplate
2. Wash Buffer
3. Stop Solution
4. Assay Diluent(s)
5. Lyophilized Standard
6. Biotinylated Detection Antibody
7. Streptavidin-Conjugated HRP
8. TMB One-Step Substrate
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Precision
Intra-Assay CV%: <10%
Inter-Assay CV%: <12%
Detection Range
0.3 ng/ml - 75 ng/ml
Sensitivity
0.3 ng/ml
Standard Curve
Principles of Testing
This assay employs an antibody specific for human FGF-20 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF-20 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FGF-20 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF-20 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Procedure
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.
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United States
Tel: 1-631-624-4882
Fax: 1-631-938-8221
Europe
Tel: 44-207-097-1828
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