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Cross-Linking and Immunoprecipitation (CLIP)

Cross-Linking and Immunoprecipitation (CLIP) is an antibody-based technique used to study RNA-protein interactions related to RNA immunoprecipitation (RIP), but differs from RIP in the use of UV radiation to cross-link RNA and the binding proteins. Unlike DNA-protein crosslinking which is done with formaldehyde, CLIP uses ultraviolet (UV) light and the crosslinking is irreversible. UV crosslinks are also more specific and only link proteins to RNAs that are in very close proximity. Further, UV crosslinks do not form between two proteins. For these reasons, UV has become the standard in RNA research. The RNA-RBP complexes are then immunoprecipitated. Due to the irreversibility of the crosslinks, the next step is digestion with a proteinase.

Creative Diagnostics can serve full line of CLIP-Seq service. We combine UV cross-linking and immunoprecipitation with high-throughput sequencing to identify binding sites of RNA-binding proteins. CLIP-seq depends on cross-linking induced mutation sites (CIMS) to localized protein-RNA binding sites. Because CIMS are reproducible, high sequencing depths allow CIMS to be differentiated from technical errors.

Besides CLIP-Seq, we also offer following services to meet your research demands.

PAR-CLIP: Photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation attempts to solve some of the problems of CLIP, namely, efficiency of crosslinking and resolution of RBP binding sites.

iCLIP: Individual-nucleotide resolution CLIP is a refinement of CLIP that allows single-nucleotide resolution of RBP binding sites.

miCLIP: Methylation individual-nucleotide-resolution crosslinking and immunoprecipitation is a specialized version of iCLIP designed to determine which RNA nucleotides are methylated by the RNA methyltransferase Nsun2.

Creative Diagnostics offers its vast expertise in CLIP to many clients in the pharmaceutical, medical device and biotech industries. We are eager to learn of our customers' specific CLIP requirement and to facilitate their research and product development.

Please feel free to contact us if you have any questions regarding our service.

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