Colloidal Gold Lateral Flow Strips Development
Creative Diagnostics offers extensive experience in the development of rapid, point-of-care, lateral-flow-format diagnostic assays. We can fully develop immunoassay test (or optimize an existing assay) according to your specifications. Once developed, we will ship the components to you, and all products associated with the project shall become the exclusive property of yours at the conclusion of the project. Full confidentiality is guaranteed.
Immunochromatography strip test, or namely lateral flow test, is a simple device intended to detect the presence or absence of the target analyte. Lateral flow test can operate as either competitive or sandwich assays. It’s a form of immunoassay in which the test sample flow along the PVDF membrane via capillary action. Probably the most well-known examples of lateral flow tests are in-home pregnancy tests.
The high speed and simplicity for testing are the advantages of lateral flow tests compared to other immunoassays. It takes only a few minutes to obtain the test results and requires little or no samples/reagents preparations.
For lateral flow test, in principle any coloured particles can be involved, and the most frequently used is nanometer sized colloid gold. Fluorescent or magnetic labeled particles can also be involved - however these require an additional electronic reader to assess the results.
Lateral flow can be applied for quantitative tests. Generally speaking, there is a trade-off between time and sensitivity – so more sensitive tests should take longer to develop. It is possible to measure the intensity of the test line to determine the quantity of target molecules in the sample. For example, implementing fluorescent or magnetic particles with paired equipment allows for getting a quantified result.
There is a wide range of applications of lateral flow including HIV tests, Troponin T tests, Malaria tests, drug of abuse tests, fertility tests, respiratory test, etc. The lateral flow tests can be applied to urine, saliva, blood, stool samples, food, or water.