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Human Anti-Tetanus Toxin Heterohybridoma [0G23]   (CSC-H1138)  


This hybridoma produces mAbs (IgG1) against Tetanus Toxin           Datasheet

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General Information
Isotype
IgG1
Fusion Species
Human X Mouse Heterohybridoma
Immunological Donor
Human
Myeloma
P3X63Ag8.653
Clone
0G23
Cell Line Description
Human peripheral blood mononuclear cells (enriched for B cells) were fused with P3X63Ag8.653 myeloma cells.
Morphology
Lymphoblast
Growth Properties
Suspension
Culture Method
Complete Growth Medium
RPMI 1640 with 2 mM L-glutamine, 1 mM sodium pyruvate, 5 mcg/ml insulin and 5 mcg/ml transferrin
, supplemented with 15% FBS
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Target
Introduction
Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.

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