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Human Anti-Human Intracellular Antigen Heterohybridoma [Uf40]   (CSC-H1051)  


This hybridoma produces mAbs (IgM) against human Intracellular antigen           Datasheet

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General Information
Isotype
IgM
Fusion Species
Human X Mouse Heterohybridoma
Immunological Donor
Human
Myeloma
NS-1
Clone
Uf40
Cell Line Description
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer. Lymphocytes were fused with NS-1 myeloma cells.
Morphology
Lymphoblast
Growth Properties
Suspension
Culture Method
Complete Growth Medium
RPMI 1640 with 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate, 0.1 mM hypoxanthine and 0.016 mM thymidine
, supplemented with 10% FBS
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.

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