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Anti-RAD50 monoclonal antibody   (CABT-35932MH)  


Mouse anti-Human RAD50 monoclonal antibody for ICC/IF, IHC-P, IP, WB           Datasheet

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Specifications
Host Species
Mouse
Antibody Isotype
IgG1
Clone
24C4/3D7
Species Reactivity
Mouse, Human
Immunogen
Fusion protein containing the complete coding region (amino acids 1-425) of RAD50 expressed in E.coli.
Conjugate
Unconjugated
Applications
Application Notes
Flow Cyt: 1μg/106 cells; IHC-P: 1/400; ICC/IF: 1/200; WB: 1/1000;

*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target
Alternative Names
RAD50; RAD50 homolog (S. cerevisiae); RAD50 (S. cerevisiae) homolog; DNA repair protein RAD50; hRad50; RAD50 2; DNA repair protein RAD50; hRad50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50_
Entrez Gene ID
10111
UniProt ID
A5D6Y3
Product Background
Pathway
Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Homologous Recombination Repair, organism-specific biosystem; Homologous recombination, organism-specific biosystem; Homologous recombination, conserved biosystem; Homo
References
Lopez-Casamichana, M; Orozco, E; et al. Transcriptional profile of the homologous recombination machinery and characterization of the EhRAD51 recombinase in response to DNA damage in Entamoeba histolytica. BMC MOLECULAR BIOLOGY 9:-(2008).
Huang, JR; Dynan, WS; et al. Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction. NUCLEIC ACIDS RESEARCH 30:667-674(2002).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.
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United States
Tel: 1-631-624-4882
Fax: 1-631-938-8221
Europe
Tel: 44-207-048-3343
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