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Anti-CENPA monoclonal antibody   (CABT-30677RH)  


Rabbit anti-Human CENPA monoclonal antibody for FC, ICC/IF, IHC-P, WB           Datasheet

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Specifications
Host Species
Rabbit
Antibody Isotype
IgG
Clone
FQ900Z
Species Reactivity
Human
Immunogen
Synthetic peptide corresponding to residues in the C-terminal of human CENPA.
Conjugate
Unconjugated
Applications
Application Notes
WB: 1/20000; ICC/IF: 1/250 - 1/500; Flow Cyt: 1/80 - 1/100;

*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target
Alternative Names
CENPA; centromere protein A; centromere protein A (17kD) ,centromere protein A, 17kDa; histone H3-like centromeric protein A; CENP A; CENP A; CENP-A; cenpa; CENPA_HUMAN; Centromere autoantigen A; Centromere protein A 17kDa; centromere protein A; centromer
Entrez Gene ID
1058
UniProt ID
P49450
Product Background
Gene Summary
CENPA (Centromere Protein A) is a Protein Coding gene. Diseases associated with CENPA include systemic scleroderma and rheumatic disease. Among its related pathways are Signaling by GPCR and Signaling by Rho GTPases. GO annotations related to this gene include protein heterodimerization activity and chromatin binding. Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Antigen Description
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro). Centromere protein A, also known as CENPA, is a protein which in humans is encoded by the CENPA gene. Centromeres are the chromosomal domains that specify the mitotic behavior of chromosomes. The CENPA gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Pathway
Aurora A signaling, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; DNA Replication, organism-specific biosystem; Deposition of New CENPA-containing Nucleosomes at the Centromere, organism-specific biosystem; FOXM1 transcription factor network, organism-specific biosystem; M Phase, organism-specific biosystem; Mitotic M-M/G1 phases, organism-specific biosystem; Mit.
References
Valdivia, MM; Hamdouch, K; et al. CENPA a Genomic Marker for Centromere Activity and Human Diseases. CURRENT GENOMICS 10:326-335(2009).
Hecker, M; Lorenz, P; et al. Computational analysis of high-density peptide microarray data with application from systemic sclerosis to multiple sclerosis. AUTOIMMUNITY REVIEWS 11:180-190(2012).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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