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Human Anti-Diphtheria Toxin Heterohybridoma [27N4G21]   (CSC-H1231)  


This hybridoma produces mAbs (IgG) against Diphtheria Toxin           Datasheet

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General Information
Isotype
IgG
Fusion Species
Human X Mouse Heterohybridoma
Immunological Donor
Human peripheral blood mononuclear
Myeloma
P3X63Ag8.653
Clone
27N4G21
Cell Line Description
Human peripheral blood mononuclear cells (enriched for B cells) were fused with P3X63Ag8.653 myeloma cells.
Morphology
Lymphoblast
Growth Properties
Suspension
Culture Method
Complete Growth Medium
RPMI 1640 with 2 mM L-glutamine, 1 mM sodium pyruvate, 5 mcg/ml insulin and 5 mcg/ml transferrin, supplemented with 15% FBS
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Target
Introduction
Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae, the pathogen bacterium that causes diphtheria. Unusually, the toxin gene is encoded by a bacteriophage (a virus that infects bacteria).The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis.

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